Similar substrate recognition motifs for mammalian AMP-activated protein kinase, higher plant HMG-CoA reductase kinase-A, yeast SNF1, and mammalian calmodulin-dependent protein kinase I

• Published in: FEBS letters Vol. 361; no. 2; pp. 191 - 195
• Main Authors: Dale, Susan, Wilson, Wayne A., Edelman, Arthur M., Hardie, D.Grahame
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 20.03.1995
• Subjects: 3-hydroxy-3-methyl; Amino Acid Sequence; AMP-activated protein kinase; AMP-Activated Protein Kinases; AMP-PK; Animals; Binding Sites; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; Calmodulin-dependent protein kinase I; CaMKI; Consensus sequence; cyclic AMP-dependent protein kinase; HMG; HMG-CoA reductase kinase; HMG-CoA reductase kinase-A; HRK—A; Mammals; Molecular Sequence Data; Multienzyme Complexes - metabolism; Oligopeptides - chemical synthesis; Oligopeptides - chemistry; Oligopeptides - metabolism; Phosphorylation; PKA; Plants - enzymology; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases - metabolism; Saccharomyces cerevisiae - metabolism; SNF; SNF1; Specificity determinant; Structure-Activity Relationship; Substrate Specificity; sucrose non-fermenting; SNF1; CaMKI; HMG; Consensus sequence; Specificity determinant; PKA; AMP-PK; SNF; AMP-activated protein kinase; HMG-CoA reductase kinase; Calmodulin-dependent protein kinase I; HRK—A
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)00172-6

We have analysed phosphorylation of the synthetic peptide AMARAASAAALARRR, and 23 variants, by mammalian, higher plant and yeast members of the SNF1 protein kinase subfamily (AMP-activated protein kinase (AMPK), HMG-CoA reductase kinase (HRK-A), and SNF1 itself), and by mammalian calmodulin-dependent protein kinase I (CaMKI). These four kinases recognize motifs which are very similar, although distinguishable. Our studies define the following recognition motifs: AMPK: Φ(X,β)XXS/TXXXΦ; HRK-A: Φ(X,β)XXSXXXΦ; Snf1: ΦXRXXSXXXΦ; CaMKI: ΦXRXXS/TXXXΦ; where Φ is a hydrophobic residue (M, V, L, I or F) and β is a basic residue (R, K or H).