Fine mapping of Co, a gene controlling columnar growth habit located on apple (Malus×domestica Borkh.) linkage group 10

• Published in: Plant breeding Vol. 131; no. 5; pp. 641 - 647
• Main Authors: Moriya, Shigeki, Okada, Kazuma, Haji, Takashi, Yamamoto, Toshiya, Abe, Kazuyuki
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.2012; Blackwell; Wiley Subscription Services, Inc
• Subjects: Agronomy. Soil science and plant productions; alleles; ancestry; apple; apple genome; apples; bin mapping; Biological and medical sciences; breeding; Classical and quantitative genetics. Population genetics. Molecular genetics; columnar growth habit; crop production; dominant genes; fine mapping; fruit growing; Fruits; Fundamental and applied biological sciences. Psychology; Gene mapping; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetics; Genetics and breeding of economic plants; Genomes; Genomics; Genotyping; Germplasm; graphical genotype; growth habit; Malus; marker-assisted selection; microsatellite repeats; Molecular genetics; Nucleotide sequence; Plant breeding; Plant material; Planting; production technology; Progeny; Recombination; Shoots; simple sequence repeat; Spreading; Trees; columnar growth habit; Cartography; Bin; Rosaceae; Genotype; Malus domestica; graphical genotype; Co; Genetic determinism; Growth habit; bin mapping; Apple; Dicotyledones; Microsatellite DNA; Linkage group; Angiospermae; Genetic improvement; Spermatophyta; Fruit tree; Genome; simple sequence repeat; fine mapping; apple genome
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2012.01985.x

With 2 figures and 3 tables ABSTRACT: Tree architecture and shoot morphology are important factors in apple production systems. Because a columnar growth habit facilitates high‐density planting and labour‐saving fruit production methods, columnar apple breeding programmes are spreading worldwide. Columnar growth habit is controlled by a single dominant gene, Co, on linkage group 10. Fine mapping of Co was conducted using 1000 F1 progeny plants from 31 populations. Novel simple sequence‐repeat (SSR) markers were developed based on the genome sequence of apple ‘Golden Delicious’ and analysed for linkage to Co. Graphical genotyping of 22 progeny with recombination event in the vicinity of Co identified three SSR markers (Mdo.chr10.12, Mdo.chr10.13 and Mdo.chr10.14) that co‐segregated with Co. Mdo.chr10.12 and Mdo.chr10.14 are well suited for marker‐assisted selection because the sizes of the alleles linked to Co are different from those found in common ancestors of current apple breeding germplasm. The Co region was delimited to a 196‐kb region of the apple genome, between markers Mdo.chr10.11 and Mdo.chr10.15.