Reduction of expression of IL‐18, IL‐1β genes in the articular joint by sumac fruit extract (Rhus coriaria L.)
Background Osteoarthritis is the most common malignant disease in the world. The disease is caused by changes in the metabolism, the structure and function of multiple joints, and joint tissues. Sumac is one of the indigenous plants of Iran and has traditionally been used as a spice in Iran. The aim...
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Published in | Molecular genetics & genomic medicine Vol. 7; no. 6; pp. e664 - n/a |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
John Wiley & Sons, Inc
01.06.2019
John Wiley and Sons Inc Wiley |
Subjects | |
Online Access | Get full text |
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Summary: | Background
Osteoarthritis is the most common malignant disease in the world. The disease is caused by changes in the metabolism, the structure and function of multiple joints, and joint tissues. Sumac is one of the indigenous plants of Iran and has traditionally been used as a spice in Iran. The aim of this study was to investigate the reduction of expression of IL‐18, IL‐1β genes in the articular joint by sumac fruit extract (Rhus coriaria L.).
Methods
The alcoholic extract of sumac fruit (E.E.R.C.L) was prepared from the Genetic Reserve Center. Bleeding was used to provide synoviocyte cells from the joint and fluid of the anatomical metacarpal limb of the 8‐month‐old Holstein healthy calf without any signs of inflammation. Using cell‐hemocytometer count, their viability was evaluated by trypan blue and after lipopolysaccharide (LPS) proliferation and injection to enhance the level of cytokines. After isolating the RNA and preparing the cDNA, RT‐PCR and PCR were performed and then, using the real‐time PCR method, the expression of the desired genes was investigated.
Results
In this study, after the expression of IL‐18 cytokines, IL‐1β increased to 100%, and following the treatment with alcoholic extract, the reduction of expression of these cytokines was 33.61% and 29.01%, respectively. The results of anti‐inflammatory effects showed that the alcoholic extract of sumac reduced the IL‐1β, IL‐18 expression in LPS‐stimulated cells.
Conclusion
Sumac fruit extract can be an effective medication for reducing pain.
Osteoarthritis is the most common malignant disease in the world. Sumac is one of the indigenous plants of Iran and has traditionally been used as a spice in Iran. The aim of this study was to investigate the reduction of expression of IL‐18, IL‐1β genes in the articular joint by sumac fruit extract (Rhus coriaria L.). |
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Bibliography: | Funding information The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the Lorestan University of Medical Sciences (grant No. LUMS: 7/93). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2324-9269 2324-9269 |
DOI: | 10.1002/mgg3.664 |