SDF-1 liposomes promote sustained cell proliferation in mouse diabetic wounds

• Published in: Wound repair and regeneration Vol. 23; no. 5; pp. 711 - 723
• Main Authors: Olekson, Melissa A. Przyborowski, Faulknor, Renea, Bandekar, Amey, Sempkowski, Michelle, Hsia, Henry C., Berthiaume, François
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.09.2015
• Subjects: Acellular Dermis; Animals; Cell Proliferation; Chemokine CXCL12 - administration & dosage; Chemotaxis; Dermis; Diabetes; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - pathology; Growth factors; Humans; Liposome; Liposomes; Mice; Skin - metabolism; Skin - pathology; Tissue Engineering; Tissue Scaffolds; Wound healing; Wound Healing - physiology; Wounds and Injuries - etiology; Wounds and Injuries - metabolism; Wounds and Injuries - therapy; Dermis; Liposome; Wound healing; Diabetes; Chemotaxis; Growth factors
• ISSN: 1067-1927; 1524-475X
• DOI: 10.1111/wrr.12334

ABSTRACT Chronic skin wounds are a common complication of diabetes. When standard wound care fails to heal such wounds, a promising approach consists of using decellularized matrices and other porous scaffold materials to promote the restoration of skin. Proper revascularization is critical for the efficacy of such materials in regenerative medicine. Stromal cell‐derived factor‐1 (SDF‐1) is a chemokine known to play a key role for angiogenesis in ischemic tissues. Herein we developed nanosized SDF‐1 liposomes, which were then incorporated into decellularized dermis scaffolds used for skin wound healing applications. SDF‐1 peptide associated with liposomes with an efficiency of 80%, and liposomes were easily dispersed throughout the acellular dermis. Acellular dermis spiked with SDF‐1 liposomes exhibited more persistent cell proliferation in the dermis, especially in CD31+ areas, compared to acellular dermis spiked with free SDF‐1, which resulted in increased improved wound closure at day 21, and increased granulation tissue thickness at day 28. SDF‐1 liposomes may increase the performance of a variety of decellularized matrices used in tissue engineering.