Molecularly imprinted polymers for bisphenol A for HPLC and SPE from water and milk

• Published in: Journal of separation science Vol. 31; no. 12; pp. 2272 - 2282
• Main Authors: Alexiadou, Despina K, Maragou, Niki C, Thomaidis, Nikolaos S, Theodoridis, Georgios A, Koupparis, Michael A
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.07.2008; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley
• Subjects: Analytical chemistry; Biological and medical sciences; Bisphenol A; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; LC-MS; Milk; Milk and cheese industries. Ice creams; Molecular Imprinting; Non alcoholic beverage industries and mineral waters; Other chromatographic methods; SPE; Solid phase extraction; Chemical analysis; Bottled water; Estrogen; HPLC chromatography; SPE; Chemical enrichment; Affinity adsorbent; Crosslinked copolymer; Sample preparation; Affinity chromatography; Quantitative analysis; Milk; Stationary phase; Trace analysis; Validation; Mimetic hormone; Coupled method; Endocrine disruptor; Bisphenol A; LC-MS; Preparation; Phenols; Methacrylate copolymer; Molecular imprinting; Mass spectrometry
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200700643

Molecularly imprinted polymers (MIPs) for bisphenol A (BPA) were prepared by two synthetic routes: semi-covalent and noncovalent methodology. The molecular imprinting effect was evaluated using the polymers in HPLC and SPE. Polymers prepared with noncovalent mode were proven more effective. These polymers were applied in SPE facilitating selective retention of BPA from bottled water and milk. The developed sample preparation was simple and efficient comprising only dilution of milk and MISPE prior to LC-MS analysis. Overall MISPE enhanced sample clean-up. Compared with control nonimprinted polymers and conventional C18 SPE cartridges, the MIPs exhibited selective analyte recognition. The method provided quantitative BPA recoveries, very good reproducibility (% RSDs lower than 7%), and low LOD (0.2 ng/g). MIP interacts similarly with deuterated BPA allowing its use as internal standard in LC-MS. The most critical parameters of MISPE were the organic content in loading-washing medium and the washing volume. Low flow rates in the elution step enhanced extraction recovery. Important advantages of the MIP were: the high breakthrough volumes (> 500 mL of water), high mass capacity (> 10 ng/mg of MIP sorbent), good linearity, and good stability in performance for over 35 cycles of use.