Molecularly imprinted polymers for bisphenol A for HPLC and SPE from water and milk

Molecularly imprinted polymers (MIPs) for bisphenol A (BPA) were prepared by two synthetic routes: semi-covalent and noncovalent methodology. The molecular imprinting effect was evaluated using the polymers in HPLC and SPE. Polymers prepared with noncovalent mode were proven more effective. These po...

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Published inJournal of separation science Vol. 31; no. 12; pp. 2272 - 2282
Main Authors Alexiadou, Despina K, Maragou, Niki C, Thomaidis, Nikolaos S, Theodoridis, Georgios A, Koupparis, Michael A
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 01.07.2008
WILEY-VCH Verlag
WILEY‐VCH Verlag
Wiley
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Summary:Molecularly imprinted polymers (MIPs) for bisphenol A (BPA) were prepared by two synthetic routes: semi-covalent and noncovalent methodology. The molecular imprinting effect was evaluated using the polymers in HPLC and SPE. Polymers prepared with noncovalent mode were proven more effective. These polymers were applied in SPE facilitating selective retention of BPA from bottled water and milk. The developed sample preparation was simple and efficient comprising only dilution of milk and MISPE prior to LC-MS analysis. Overall MISPE enhanced sample clean-up. Compared with control nonimprinted polymers and conventional C18 SPE cartridges, the MIPs exhibited selective analyte recognition. The method provided quantitative BPA recoveries, very good reproducibility (% RSDs lower than 7%), and low LOD (0.2 ng/g). MIP interacts similarly with deuterated BPA allowing its use as internal standard in LC-MS. The most critical parameters of MISPE were the organic content in loading-washing medium and the washing volume. Low flow rates in the elution step enhanced extraction recovery. Important advantages of the MIP were: the high breakthrough volumes (> 500 mL of water), high mass capacity (> 10 ng/mg of MIP sorbent), good linearity, and good stability in performance for over 35 cycles of use.
Bibliography:http://dx.doi.org/10.1002/jssc.200700643
Greek State Scholarships Foundation
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ArticleID:JSSC200700643
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ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200700643