Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum

• Published in: MicrobiologyOpen (Weinheim) Vol. 8; no. 12; pp. e911 - n/a
• Main Authors: Djami‐Tchatchou, Arnaud T., Matsaunyane, Lerato B. T., Ntushelo, Khayalethu
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.12.2019; John Wiley and Sons Inc; Wiley
• Subjects: Ammonia; Chitinase; Cytochrome; Cytochrome P450; Cytochromes P450; defense; Deoxyribonucleic acid; DNA; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Guanosine triphosphatases; Host-Pathogen Interactions - genetics; Lycopersicon esculentum - genetics; Lycopersicon esculentum - microbiology; Original; Pathogenesis; Pathogens; Pectobacterium carotovorum; Pectobacterium carotovorum subsp. carotovorum; Phenylalanine; Plant Diseases - genetics; Plant Diseases - microbiology; Plant resistance; Proteinase; Proteinase inhibitors; Proteins; Soft rot; Software; Studies; tomato; Tomatoes; Transcriptome; Pectobacterium carotovorum subsp. carotovorum; tomato; defense; gene expression
• ISSN: 2045-8827; 2045-8827
• DOI: 10.1002/mbo3.911

Defense responses of tomato (Solanum lycopersicum L.) against attack by Pectobacterium carotovorum subsp. carotovorum (Pcc), the causal agent of soft rot diseases, were studied. The expression of some tomato defense genes were evaluated by real‐time PCR quantification analysis, 24 and 72 hr after actively growing tomato plants were inoculated with Pcc. These included: MYB transcriptor factor, ethylene response element‐binding protein, suppressor of the G2 allele of Skp1, cytochrome P450, small Sar1 GTPase, hydroxycinnamoyl‐CoA:quinate hydroxycinnamoyl transferase, pathogenesis‐related protein 1a, endo‐1,3‐beta‐glucanase, chitinase, proteinase inhibitor, defensin, CC‐NBS‐LRR resistance protein, and phenylalanine ammonia lyase. The results showed dynamic transcriptomic changes, with transcripts exhibiting different expression kinetics at 24 and 72 hr to confer resistance to tomato against Pcc infection. Targeting various disease response‐related genes, real‐time PCR was performed on tomato leaf tissue sampled 24 and 72 hr postinoculation with Pectobacterium carotovorum subsp. carotovorum (Pcc). The results showed dynamic transcriptomic changes, with transcripts exhibiting different expression kinetics at 24 and 72 hr to confer resistance to tomato against Pcc infection.