Equilibrium self-association of tropomyosin

► Skeletal muscle tropomyosin is in equilibrium between monomer, dimer and tetramer. ► Tropomyosin species type determines tropomyosin-actin binding kinetics. ► Analytical ultracentrifugation is an ideal technique to address tropomyosin dynamics. It has recently been reported that tropomyosin exists...

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Bibliographic Details
Published inFEBS letters Vol. 586; no. 21; pp. 3840 - 3842
Main Authors Stafford, Walter F., Lee, Eunhee, Graceffa, Philip
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 02.11.2012
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Summary:► Skeletal muscle tropomyosin is in equilibrium between monomer, dimer and tetramer. ► Tropomyosin species type determines tropomyosin-actin binding kinetics. ► Analytical ultracentrifugation is an ideal technique to address tropomyosin dynamics. It has recently been reported that tropomyosin exists exclusively as a dimer in physiological salt conditions. It is shown in the present work using analytical ultracentrifugation that, on the contrary, tropomyosin is in equilibrium between monomer, dimer and tetramer with a weak tendency to dimerize and tetramerize. Such a finding has consequences for the assembly of the tropomyosin–actin complex.
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ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2012.08.035