The localization of the alkaloids in Coptis chinensis rhizome by time-of-flight secondary ion mass spectrometry

Understanding the spatial distribution of active compounds can effectively evaluate the quality of decoction pieces of traditional Chinese medicine (TCM). Traditional methods are economical and practical but lack chemical information on the original distribution. Time-of-flight secondary ion mass sp...

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Published inFrontiers in plant science Vol. 13; p. 1092643
Main Authors He, Fan, Huang, Yu-Feng, Dai, Wei, Qu, Xian-You, Lu, Jing-Guang, Lao, Chi-Chou, Luo, Wen-Hui, Sun, Dong-Mei, Wei, Mei, Xiao, Sheng-Yuan, Xie, Ying, Liu, Liang, Zhou, Hua
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 23.12.2022
Subjects
alkaloids
Coptis rhizome
localization
Plant Science
TOF-SIMS
UPLC-QQQ-MS/MS
localization
UPLC-QQQ-MS/MS
Coptis rhizome
TOF-SIMS
alkaloids
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Summary:Understanding the spatial distribution of active compounds can effectively evaluate the quality of decoction pieces of traditional Chinese medicine (TCM). Traditional methods are economical and practical but lack chemical information on the original distribution. Time-of-flight secondary ion mass spectrometry (TOF-SIMS), with the advantage of non-destructive detection of samples, can directly analyze the distribution of chemical compounds on the surface of various samples. In this study, TOF-SIMS image analysis technology was used to detect TCM for the first time. Taking Coptis rhizome (CR) as an example, a commonly used TCM, the distribution of the compounds in the cross-section of CR was studied. Meanwhile, ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLCQQQ-MS/MS) was used to verify the results of TOF-SIMS. The distribution of nine active compounds: berberine, epiberberine, coptisine, palmatine, columbamine, jatrorrhizine, tetrahydricheilanthifolinium, and oxyberberine, was well imaged in the cross-section of CR by TOF-SIMS. The content of berberine and epiberberine was the highest; Palmatine distribution in the pith was more than that in other parts; Oxyberberine was mainly concentrated in the cork and xylem rays. Normalization analysis showed contents of these compounds increased along with the growth years. The result was consistent with UPLC-QQQ-MS/MS. The TOF-SIMS method can display the spatial distribution status of the active compounds of herbs, providing a basis for selecting the medicine site with non-destructive and fast detection.
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This article was submitted to Plant Metabolism and Chemodiversity, a section of the journal Frontiers in Plant Science
Reviewed by: Dandan Kong, Chinese Academy of Medical Sciences, China; Hui Zhang, Liaoning University of Traditional Chinese Medicine, China; Gang Fan, Chengdu University of Traditional Chinese Medicine, China; Hui Li, Shanghai University of Traditional Chinese Medicine, China
These authors have contributed equally to this work
These authors share first authorship
Edited by: Xiongjie Zheng, King Abdullah University of Science and Technology, Saudi Arabia
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.1092643