Elastomeric electrospun scaffolds of poly(l-lactide-co-trimethylene carbonate) for myocardial tissue engineering

• Published in: Journal of materials science. Materials in medicine Vol. 22; no. 7; pp. 1689 - 1699
• Main Authors: Mukherjee, Shayanti, Gualandi, Chiara, Focarete, Maria Letizia, Ravichandran, Rajeswari, Venugopal, Jayarama Reddy, Raghunath, Michael, Ramakrishna, Seeram
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.07.2011; Springer; Springer Nature B.V
• Subjects: Animals; Biocompatible Materials - chemistry; Bioengineering; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Biotechnology; Carbonates; Cell Proliferation; Ceramics; Chemistry and Materials Science; Composites; Elastomers - chemistry; Electrospinning; Fundamental and applied biological sciences. Psychology; Glass; Health. Pharmaceutical industry; Heart; Industrial applications and implications. Economical aspects; Materials Science; Microscopy, Acoustic; Miscellaneous; Myocytes, Cardiac - cytology; Myocytes, Cardiac - physiology; Natural Materials; Polyesters - chemistry; Polymer Sciences; Polymers; Preserves; Rabbits; Regenerative Medicine/Tissue Engineering; Scaffolds; Stress-strain relationships; Surfaces and Interfaces; Thin Films; Tissue engineering; Tissue Engineering - methods; Synthetic Biomaterial; Cold Crystallization; Nanofibrous Scaffold; Electrospun Scaffold; Cardiomyocyte Proliferation; Lactone copolymer; Tissue engineering; Lactic acid copolymer; Electrospinning; Myocardium; Circulatory system; Scaffold; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-011-4351-2

In myocardial tissue engineering the use of synthetically bioengineered flexible patches implanted in the infarcted area is considered one of the promising strategy for cardiac repair. In this work the potentialities of a biomimetic electrospun scaffold made of a commercial copolymer of ( l )-lactic acid with trimethylene carbonate (P( l )LA- co -TMC) are investigated in comparison to electrospun poly( l )lactic acid. The P( l )LA- co -TMC scaffold used in this work is a glassy rigid material at room temperature while it is a rubbery soft material at 37°C. Mechanical characterization results (tensile stress–strain and creep-recovery measurements) show that at 37°C electrospun P( l )LA- co -TMC displays an elastic modulus of around 20 MPa and the ability to completely recover up to 10% of deformation. Cell culture experiments show that P( l )LA- co -TMC scaffold promotes cardiomyocyte proliferation and efficiently preserve cell morphology, without hampering expression of sarcomeric alpha actinin marker, thus demonstrating its potentialities as synthetic biomaterial for myocardial tissue engineering.