Optimization of Single-Dose VSV-Based COVID-19 Vaccination in Hamsters

• Published in: Frontiers in immunology Vol. 12; p. 788235
• Main Authors: O'Donnell, Kyle L, Clancy, Chad S, Griffin, Amanda J, Shifflett, Kyle, Gourdine, Tylisha, Thomas, Tina, Long, Carrie M, Furuyama, Wakako, Marzi, Andrea
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 06.01.2022
• Subjects: Animals; Antibodies, Viral - blood; Antibodies, Viral - immunology; Chlorocebus aethiops; COVID-19 - blood; COVID-19 - immunology; COVID-19 - prevention & control; COVID-19 - virology; COVID-19 Vaccines - administration & dosage; COVID-19 Vaccines - immunology; Cricetinae; Disease Models, Animal; Ebolavirus - genetics; Ebolavirus - immunology; Female; Humans; Immunogenicity, Vaccine; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunology; Male; Pandemics - prevention & control; Plasmids; rVSV-ZEBOV GP; SARS-CoV-2; SARS-CoV-2 - immunology; severe acute respiratory syndrome coronavirus-2; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; T-Lymphocytes - immunology; Treatment Outcome; Vaccination - methods; Vero Cells; Vesicular stomatitis Indiana virus - genetics; Vesicular stomatitis Indiana virus - immunology; vesicular stomatitis virus; VSV-EBOV; VSV-SARS2; rVSV-ZEBOV GP; SARS-CoV-2; severe acute respiratory syndrome coronavirus-2; VSV-EBOV; VSV-SARS2; vesicular stomatitis virus
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.788235

The ongoing COVID-19 pandemic has resulted in global effects on human health, economic stability, and social norms. The emergence of viral variants raises concerns about the efficacy of existing vaccines and highlights the continued need for the development of efficient, fast-acting, and cost-effective vaccines. Here, we demonstrate the immunogenicity and protective efficacy of two vesicular stomatitis virus (VSV)-based vaccines encoding the SARS-CoV-2 spike protein either alone (VSV-SARS2) or in combination with the Ebola virus glycoprotein (VSV-SARS2-EBOV). Intranasally vaccinated hamsters showed an early CD8 T cell response in the lungs and a greater antigen-specific IgG response, while intramuscularly vaccinated hamsters had an early CD4 T cell and NK cell response. Intranasal vaccination resulted in protection within 10 days with hamsters not showing clinical signs of pneumonia when challenged with three different SARS-CoV-2 variants. This data demonstrates that VSV-based vaccines are viable single-dose, fast-acting vaccine candidates that are protective from COVID-19.