Immunosensors for Pesticide Analysis: Antibody Production and Sensor Development

• Published in: Critical reviews in biotechnology Vol. 22; no. 1; pp. 15 - 32
• Main Authors: Suri, C. Raman, Raje, Manoj, Varshney, Grish C.
• Format: Journal Article
• Language: English
• Published: Boca Raton, FL Informa UK Ltd 2002; Taylor & Francis; CRC Press
• Subjects: Antibodies; antibody; Antigen-Antibody Reactions; Antigens; Biological and medical sciences; Biosensing Techniques; Biosensors; Biotechnology; Electrochemistry; Equipment Design; Fundamental and applied biological sciences. Psychology; immune-complex; immunosensor; Methods. Procedures. Technologies; Pesticides; Pesticides - analysis; Pesticides - immunology; Transducers; Various methods and equipments; Review; Antibody; Biosensor; Immunosensor; Pesticides; Production
• ISSN: 0738-8551; 1549-7801
• DOI: 10.1080/07388550290789441

Immunosensors, a type of affinity biosensor, are based on the binding interactions between an immobilized biomolecule (antibody antigen) on the electronic transducer surface with the analyte of interest (antigen antibody), resulting in a detectable signal. The sensor system takes advantage of the high selectivity provided by the molecular recognition characteristic of an antibody, which binds reversibly with a specific antigen. This review article presents the current status of immunosensors, highlighting their potential benefits and limitations for pesticide analysis. The basic criteria for generating specific antibodies against low-molecular-mass pesticides, which are usually nonimmunogenic in nature, are briefly discussed. The article also describes the fundamentals of important transducer technologies and their use in immunosensor development.