SALM Synaptic Cell Adhesion-like Molecules Regulate the Differentiation of Excitatory Synapses

Synaptic cell adhesion molecules (CAMs) are known to play key roles in various aspects of synaptic structures and functions, including early differentiation, maintenance, and plasticity. We herein report the identification of a family of cell adhesion-like molecules termed SALM that interacts with t...

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Published inNeuron (Cambridge, Mass.) Vol. 50; no. 2; pp. 233 - 245
Main Authors Ko, Jaewon, Kim, Seho, Chung, Hye Sun, Kim, Karam, Han, Kihoon, Kim, Hyun, Jun, Heejung, Kaang, Bong-Kiun, Kim, Eunjoon
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 20.04.2006
Elsevier Limited
Subjects
Animals
Blotting, Northern
Brain - physiology
Cell adhesion & migration
CELLBIO
Cells, Cultured
Dendritic Spines - metabolism
DEVBIO
Experiments
Humans
Image Processing, Computer-Assisted
In Situ Hybridization
Intracellular Signaling Peptides and Proteins - metabolism
Membrane Proteins - metabolism
MOLNEURO
Neural Cell Adhesion Molecules - genetics
Neural Cell Adhesion Molecules - metabolism
Neuronal Plasticity - physiology
Neurons
Patch-Clamp Techniques
Protein Isoforms - metabolism
Proteins
Rats
RNA, Messenger - analysis
Synapses - metabolism
Transfection
Yeast
CELLBIO
DEVBIO
MOLNEURO
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Summary:Synaptic cell adhesion molecules (CAMs) are known to play key roles in various aspects of synaptic structures and functions, including early differentiation, maintenance, and plasticity. We herein report the identification of a family of cell adhesion-like molecules termed SALM that interacts with the abundant postsynaptic density (PSD) protein PSD-95. SALM2, a SALM isoform, distributes to excitatory, but not inhibitory, synaptic sites. Overexpression of SALM2 increases the number of excitatory synapses and dendritic spines. Mislocalized expression of SALM2 disrupts excitatory synapses and dendritic spines. Bead-induced direct aggregation of SALM2 results in coclustering of PSD-95 and other postsynaptic proteins, including GKAP and AMPA receptors. Knockdown of SALM2 by RNA interference reduces the number of excitatory synapses and dendritic spines and the frequency, but not amplitude, of miniature excitatory postsynaptic currents. These results suggest that SALM2 is an important regulator of the differentiation of excitatory synapses.
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ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2006.04.005