Regulation of the Gene for Estrogenic 17-Ketosteroid Reductase Lying on Chromosome 17cen→q25

• Published in: The Journal of biological chemistry Vol. 264; no. 34; pp. 20458 - 20462
• Main Authors: Tremblay, Y, Ringler, G.E., Morel, Y, Mohandas, T.K., Labrie, F, Strauss, J.F., Miller, W.L.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.12.1989; American Society for Biochemistry and Molecular Biology
• Subjects: 17-Hydroxysteroid Dehydrogenases - genetics; Animals; Biological and medical sciences; Blotting, Southern; Cell Line; Chromosome Mapping; Chromosomes, Human, Pair 17; DNA - genetics; Estradiol Dehydrogenases - genetics; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Enzymologic; Genes; Granulosa Cells - enzymology; Humans; Hybrid Cells - enzymology; Lymphocytes - enzymology; Mice; Molecular and cellular biology; Molecular genetics; Restriction Mapping; RNA, Messenger - analysis; RNA, Messenger - genetics; Transcription, Genetic; Human; Northern blotting; Regulation(control); Hybrid cell; Gene expression; Localization; Southern blotting; Comparative study
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(19)47083-X

17-Ketosteroid reductase (17KSR), also known as 17β-hydroxysteroid dehydrogenase, catalyzes the reversible interconversion of estradiol to estrone and of androstenedione to testosterone. Using a recently cloned human placental 17KSR cDNA, we show that the 1.4-kilobase mRNA for this enzyme is detected only in tissues producing estrogens, and a 2.4-kilobase mRNA is detected in some estrogenic tissues and some androgenic tissues. This tissue distribution suggests that the interconversion of androstenedione and testosterone may be mediated by a different enzyme. Southern blotting studies show that the mRNA for this estrogenic 17KSR is encoded by two very similar genes localized to chromosome 17cenβq25 by analysis of DNA from mouse/human somatic hybrid cell lines. 8-Br-cAMP increases the abundance of estrogenic 17KSR mRNA as well as mRNAs for other steroidogenic enzymes in JEG-3 choriocarcinoma cells. By contrast, cAMP decreases estrogenic 17KSR mRNA in primary cultures of human cytotrophoblasts and human granulosa cells, a pattern of tropic regulation that differs from other steroidogenic enzyme mRNAs.