Phylogenetic Similarity of the Canine Parvovirus Wild-Type Isolates on the Basis of VP1/VP2 Gene Fragment Sequence Analysis
Biological material was taken from dogs with diarrhoea. Faecal samples were taken from within live animals and intestinal tract fragments (i.e. small intestine, and stomach) were taken from dead animals. In total, 18 specimens were investigated from dogs housed alone or in large groups. To test for...
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Published in | Journal of veterinary medicine. Series B Vol. 49; no. 3; pp. 142 - 145 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Berlin, Germany
Blackwell Verlag GmbH
01.04.2002
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Subjects | |
Online Access | Get full text |
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Summary: | Biological material was taken from dogs with diarrhoea. Faecal samples were taken from within live animals and intestinal tract fragments (i.e. small intestine, and stomach) were taken from dead animals. In total, 18 specimens were investigated from dogs housed alone or in large groups. To test for the presence of the virus, latex (On Site Biotech, Uppsala, Sweden) and direct immunofluorescence tests were performed. At the same time, polymerase chain reaction (PCR) with primers complementary to a conservative region of VP1/VP2 was carried out. The products of amplification were analysed on 2% agarose gel. The purified products were cloned with the Template Generation System (Finnzymes, Espoo, Finland) using a transposition reaction and positive clones were searched using the `colony screening by PCR' method. The sequencing gave 12 sequences of VP1/VP2 gene fragments that were of high similarity. Among the 12 analysed sequences, six exhibited 88% similarity, four exhibited 100% similarity and two exhibited 71% similarity. |
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Bibliography: | ArticleID:ZPH523 istex:0CE2AA897C1B394F105096F4087C2825D67EDBDC ark:/67375/WNG-PC16JKKG-3 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0931-1793 1439-0450 |
DOI: | 10.1046/j.1439-0450.2002.00523.x |