Protective effects of PEP-1-Catalase on stress-induced cellular toxicity and MPTP-induced Parkinson's disease

• Published in: BMB reports Vol. 48; no. 7; pp. 395 - 400
• Main Authors: Eom, S.A., Hallym University, Chunchon, Republic of Korea, Kim, D.W., Gangnung-Wonju National University, Gangneung, Republic of Korea, Shin, M.J., Hallym University, Chunchon, Republic of Korea, Ahn, E.H., Hallym University, Chunchon, Republic of Korea, Chung, S.Y., Hallym University, Chunchon, Republic of Korea, Sohn, E.J., Hallym University, Chunchon, Republic of Korea, Jo, H.S., Hallym University, Chunchon, Republic of Korea, Jeon, S.J., Soonchunhyang University, Cheonan, Republic of Korea, Kim, D.S., Soonchunhyang University, Cheonan, Republic of Korea, Kwon, H.Y., Hallym University, Chunchon, Republic of Korea, Cho, S.W., University of Ulsan College of Medicine, Seoul, Republic of Korea, Han, K.H., Hallym University, Chunchon, Republic of Korea, Park, J., Hallym University, Chunchon, Republic of Korea, Eum, W.S., Hallym University, Chunchon, Republic of Korea, Choi, S.Y., Hallym University, Chunchon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 31.07.2015; 생화학분자생물학회
• Subjects: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; Animals; Brain - drug effects; Brain - metabolism; Cell Death - drug effects; Cell Line; Cell Survival - drug effects; Disease Models, Animal; DISEASES; Dopaminergic Neurons - drug effects; Dopaminergic Neurons - metabolism; Dopaminergic Neurons - pathology; ENFERMEDAD; Humans; MALADIES; Male; Mice, Inbred C57BL; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; Parkinson Disease - drug therapy; Parkinson Disease - pathology; Parkinson's disease,ROS,PEP-1-Catalase,Protein therapy,Dopaminergic neuron; Recombinant Fusion Proteins - isolation & purification; Recombinant Fusion Proteins - pharmacology; Recombinant Fusion Proteins - therapeutic use; Signal Transduction - drug effects; Stress, Physiological - drug effects; Transduction, Genetic; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/bmbrep.2015.48.7.197

Parkinson's disease (PD) is a neurodegenerative disability caused by a decrease of dopaminergic neurons in the substantia nigra (SN). Although the etiology of PD is not clear, oxidative stress is believed to lead to PD. Catalase is antioxidant enzyme which plays an active role in cells as a reactive oxygen species (ROS) scavenger. Thus, we investigated whether PEP-1-Catalase protects against 1-methyl-4-phenylpyridinium (MPP+) induced SH-SY5Y neuronal cell death and in a 1-methyl- 4-phenyl-1,2,3,6-trtrahydropyridine (MPTP) induced PD animal model. PEP-1-Catalase transduced into SH-SY5Y cells significantly protecting them against MPP+-induced death by decreasing ROS and regulating cellular survival signals including Akt, Bax, Bcl-2, and p38. Immunohistochemical analysis showed that transduced PEP-1-Catalase markedly protected against neuronal cell death in the SN in the PD animal model. Our results indicate that PEP-1-Catalase may have potential as a therapeutic agent for PD and other oxidative stress related diseases.