Isolation of Flat Revertants from Human Papillomavirus Type 18 E6E7 Transformed 3Y1 Cells by Transfection with a Rat Embryo Fibroblast cDNA Expression Library

A rat embryo fibroblast (REF) cDNA expression library was transfected into 3Y1 cells transformed by human papillomavirus type 18 E6 and E7 genes and 10 flat revertants were isolated. These revertants expressed the same levels of E6 and E7 mRNA as the parent cells, but had greatly reduced ability to...

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Published inCell Structure and Function Vol. 18; no. 6; pp. 457 - 465
Main Authors Nakanishi, Kazuyoshi, Yong-Il, Hwang, Ishiwatari, Haruko, Takami, Yasunari, Hayasaka, Naoto, Yutsudo, Masuo, Nojima, Hiroshi, Hakura, Akira
Format Journal Article Conference Proceeding
LanguageEnglish
Published Kyoto Japan Society for Cell Biology 01.12.1993
Japan Science and Technology Agency
Subjects
Animals
Biological and medical sciences
Biotechnology
cDNA expression library
Cell Separation
Cell Survival - drug effects
Cell Transformation, Viral - genetics
DNA, Complementary - genetics
Embryo, Mammalian - physiology
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
Gene Library
Genes, Tumor Suppressor
Genetic engineering
Genetic technics
Genetic Vectors
human papillomavirus
Hydroxyproline - pharmacology
Methods. Procedures. Technologies
Miscellaneous
Mutation
Papillomaviridae
Rats
revertants
Stereoisomerism
Synthetic digonucleotides and genes. Sequencing
Transfection - genetics
transformation
Papillomavirus
Virus
Human papillomavirus
Transfection
Complementary DNA
Transformed cell
Papovaviridae
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Summary:A rat embryo fibroblast (REF) cDNA expression library was transfected into 3Y1 cells transformed by human papillomavirus type 18 E6 and E7 genes and 10 flat revertants were isolated. These revertants expressed the same levels of E6 and E7 mRNA as the parent cells, but had greatly reduced ability to form colonies in soft agar. Suppression of transformation was dominant in cell hybrids generated by fusing each revertant with the parental transformed cells. Furthermore, loss of transfected cDNA was observed in re-transformed cell hybrids derived from one flat revertant. Over expression of the CDNA suppresses the colony-forming efficiency of the cells transformed by E6 and E7 genes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0386-7196
1347-3700
DOI:10.1247/csf.18.457