Inhibition of the Membrane Attack Complex by Dengue Virus NS1 through Interaction with Vitronectin and Terminal Complement Proteins

• Published in: Journal of virology Vol. 90; no. 21; pp. 9570 - 9581
• Main Authors: Conde, Jonas Nascimento, da Silva, Emiliana Mandarano, Allonso, Diego, Coelho, Diego Rodrigues, Andrade, Iamara da Silva, de Medeiros, Luciano Neves, Menezes, Joice Lima, Barbosa, Angela Silva, Mohana-Borges, Ronaldo
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.11.2016
• Subjects: Cell Line, Tumor; Complement Membrane Attack Complex - metabolism; Complement System Proteins - metabolism; Dengue - metabolism; Dengue - virology; Dengue Virus - metabolism; Flaviviridae; Flavivirus; Flavivirus - metabolism; Humans; Protein Binding - physiology; Two-Hybrid System Techniques; Viral Nonstructural Proteins - metabolism; Virus-Cell Interactions; Vitronectin - metabolism; West Nile virus; West Nile virus - metabolism; Zika virus; Zika Virus - metabolism; Zika Virus Infection - metabolism; Zika Virus Infection - virology
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/JVI.00912-16

Dengue virus (DENV) infects millions of people worldwide and is a major public health problem. DENV nonstructural protein 1 (NS1) is a conserved glycoprotein that associates with membranes and is also secreted into the plasma in DENV-infected patients. The present study describes a novel mechanism by which NS1 inhibits the terminal complement pathway. We first identified the terminal complement regulator vitronectin (VN) as a novel DENV2 NS1 binding partner by using a yeast two-hybrid system. This interaction was further assessed by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) assay. The NS1-VN complex was also detected in plasmas from DENV-infected patients, suggesting that this interaction occurs during DENV infection. We also demonstrated that the DENV2 NS1 protein, either by itself or by interacting with VN, hinders the formation of the membrane attack complex (MAC) and C9 polymerization. Finally, we showed that DENV2, West Nile virus (WNV), and Zika virus (ZIKV) NS1 proteins produced in mammalian cells inhibited C9 polymerization. Taken together, our results points to a role for NS1 as a terminal pathway inhibitor of the complement system. Dengue is the most important arthropod-borne viral disease nowadays and is caused by dengue virus (DENV). The flavivirus NS1 glycoprotein has been characterized functionally as a complement evasion protein that can attenuate the activation of the classical, lectin, and alternative pathways. The present study describes a novel mechanism by which DENV NS1 inhibits the terminal complement pathway. We identified the terminal complement regulator vitronectin (VN) as a novel DENV NS1 binding partner, and the NS1-VN complex was detected in plasmas from DENV-infected patients, suggesting that this interaction occurs during DENV infection. We also demonstrated that the NS1-VN complex inhibited membrane attack complex (MAC) formation, thus interfering with the complement terminal pathway. Interestingly, NS1 itself also inhibited MAC activity, suggesting a direct role of this protein in the inhibition process. Our findings imply a role for NS1 as a terminal pathway inhibitor of the complement system.