Comparison of Three Molecular Methods Used for Subtyping of Legionella pneumophila Strains Isolated during an Epidemic of Legionellosis in Rome
In the summer of 2003 a community-acquired outbreak of Legionella pneumophila occurred in Rome, Italy. Three molecular typing methods, pulse-field gel electrophoresis, amplified fragment length polymorphism analysis, and sequence-based typing (SBT), were used to establish the clonal correlation amon...
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Published in | Journal of Clinical Microbiology Vol. 43; no. 10; pp. 5348 - 5350 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Society for Microbiology
01.10.2005
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Subjects | |
Online Access | Get full text |
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Summary: | In the summer of 2003 a community-acquired outbreak of Legionella pneumophila occurred in Rome, Italy. Three molecular typing methods, pulse-field gel electrophoresis, amplified fragment length polymorphism analysis, and sequence-based typing (SBT), were used to establish the clonal correlation among the isolates of the epidemic cluster. By comparison of the methods, SBT was the most rapid and the easiest to perform and provided unambiguous results. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Department of Infectious Parasitic and Immunomediate Diseases, Istituto Superiore di Sanità, Viale Regina Elena, 299-00161 Rome, Italy. Phone: 390649902856. Fax: 390649387112. E-mail: marisa.scaturro@iss.it. |
ISSN: | 0095-1137 1098-660X |
DOI: | 10.1128/JCM.43.10.5348-4350.2005 |