Comparison of Three Molecular Methods Used for Subtyping of Legionella pneumophila Strains Isolated during an Epidemic of Legionellosis in Rome

In the summer of 2003 a community-acquired outbreak of Legionella pneumophila occurred in Rome, Italy. Three molecular typing methods, pulse-field gel electrophoresis, amplified fragment length polymorphism analysis, and sequence-based typing (SBT), were used to establish the clonal correlation amon...

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Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 43; no. 10; pp. 5348 - 5350
Main Authors Scaturro, M, Losardo, M, De Ponte, G, Ricci, M. L
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.10.2005
Subjects
amplified fragment length polymorphism
bacterial infections
Bacterial Proteins - genetics
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Disease Outbreaks
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
human diseases
Humans
Infectious diseases
Italy - epidemiology
Legionella pneumophila
Legionella pneumophila - classification
Legionella pneumophila - genetics
Legionella pneumophila - isolation & purification
Legionnaires' Disease - epidemiology
Legionnaires' Disease - microbiology
Medical sciences
Microbiology
Miscellaneous
pathogen identification
Polymorphism, Restriction Fragment Length
pulsed-field gel electrophoresis
Sequence Analysis, DNA
strains
Italy
Infection
Epidemic
Legionellaceae
Microbiology
Bacteriosis
Bacteria
Legionella pneumophila
Legionellosis
Method
Strain
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Summary:In the summer of 2003 a community-acquired outbreak of Legionella pneumophila occurred in Rome, Italy. Three molecular typing methods, pulse-field gel electrophoresis, amplified fragment length polymorphism analysis, and sequence-based typing (SBT), were used to establish the clonal correlation among the isolates of the epidemic cluster. By comparison of the methods, SBT was the most rapid and the easiest to perform and provided unambiguous results.
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Corresponding author. Mailing address: Department of Infectious Parasitic and Immunomediate Diseases, Istituto Superiore di Sanità, Viale Regina Elena, 299-00161 Rome, Italy. Phone: 390649902856. Fax: 390649387112. E-mail: marisa.scaturro@iss.it.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.10.5348-4350.2005