Silver nanoparticles synthesized from Pseudomonas aeruginosa pyoverdine: Antibiofilm and antivirulence agents

• Published in: Biofilm Vol. 7; p. 100192
• Main Authors: Tabassum, Nazia, Khan, Fazlurrahman, Jeong, Geum-Jae, Jo, Du-Min, Kim, Young-Mog
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2024; Elsevier
• Subjects: Antibiofilm; Antivirulence; Host-mimicking media; Pyoverdine; Silver nanoparticles; Antivirulence; Antibiofilm; Pyoverdine; Silver nanoparticles; Host-mimicking media
• ISSN: 2590-2075; 2590-2075
• DOI: 10.1016/j.bioflm.2024.100192

The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one of the major issues in the healthcare sector. In the present study, nanomaterial-based controlling the biofilm and virulence properties has been considered an alternative approach. Pyoverdine (PVD) isolated from the Pseudomonas aeruginosa was utilized as a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful in a targeted action to microbial pathogens due to the recognition of the corona of the nanoparticles by the pathogenic membrane. Synthesized PVD-AgNPs were spherical to irregular, with an average size value of 251.87 ± 21.8 nm and zeta potential with a value of −36.51 ± 0.69 mV. The MIC value of PVD-AgNPs towards P. aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, and Candida albicans in the standard and host-mimicking media were observed in decreasing order in a multi-fold, such as standard growth media > sputum > synthetic human urine > saliva. Both the initial stage and the well-established biofilms of these microbial pathogens have been effectively inhibited and eradicated by PVD-AgNPs. PVD-AgNPs increase the susceptibility of tetracycline, PVD, and amphotericin B towards established mature mono- and mixed-species biofilms of S. aureus and C. albicans. Additionally, PVD-AgNPs attenuate several virulence properties, such as inhibition of protease activity, motility, and PVD and pyocyanin production in P. aeruginosa. The inhibition of gene expression of biofilm and virulence-associated genes in P. aeruginosa validates its phenotypic effects. •Pyoverdine from Pseudomonas aeruginosa was used as a biological corona to synthesize AgNPs.•PVD-AgNPs effectively inhibited and eradicated initial stage and mature biofilms.•PVD-AgNPs increased the susceptibility of tetracycline and amphotericin B towards mature biofilm.•PVD-AgNPs attenuated several virulence properties in P. aeruginosa.•The suppression of genes associated with biofilm and virulence validates its phenotypic effects.