Genetic mapping of a putative Thinopyrum intermedium-derived stripe rust resistance gene on wheat chromosome 1B

• Published in: Theoretical and applied genetics Vol. 127; no. 4; pp. 843 - 853
• Main Authors: Huang, Q, Li, X, Chen, W. Q, Xiang, Z. P, Zhong, S. F, Chang, Z. J, Zhang, M, Zhang, H. Y, Tan, F. Q, Ren, Z. L, Luo, P. G
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2014; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Agricultural research; Agriculture; Basidiomycota - physiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; chromosome mapping; Chromosome Mapping - methods; Chromosomes; Chromosomes, Plant - genetics; Crosses, Genetic; Disease Resistance - genetics; Diseases and pests; dominant genes; Electrophoresis, Polyacrylamide Gel; Gene expression; Genes; Genes, Plant; Genetic aspects; Genetic Markers; Genetic research; Health aspects; Inheritance Patterns - genetics; Life Sciences; marker-assisted selection; microsatellite repeats; Microsatellite Repeats - genetics; Original Paper; Phenotype; Plant Biochemistry; Plant Breeding/Biotechnology; Plant diseases; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Plant Leaves - genetics; Plant Leaves - microbiology; Poaceae - genetics; Puccinia striiformis; reciprocal crosses; Rust fungi; Silver Staining; Thinopyrum intermedium; Triticum - genetics; Triticum - microbiology; Triticum aestivum; Virulence; Wheat; China; Stripe Rust; Wheat Chromosome; Simple Sequence Repeat Marker; Fusarium Head Blight; Powdery Mildew
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-014-2261-7

KEY MESSAGE : Stripe rust resistance transferred from Thinopyrum intermedium into common wheat was controlled by a single dominant gene, which mapped to chromosome 1B near Yr26 and was designated YrL693. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a highly destructive disease of wheat (Triticum aestivum). Stripe rust resistance was transferred from Thinopyrum intermedium to common wheat, and the resulting introgression line (L693) exhibited all-stage resistance to the widely virulent and predominant Chinese pathotypes CYR32 and CYR33 and to the new virulent pathotype V26. There was no cytological evidence that L693 had alien chromosomal segments from Th. intermedium. Genetic analysis of stripe rust resistance was performed by crossing L693 with the susceptible line L661. F₁, F₂, and F₂:₃ populations from reciprocal crosses showed that resistance was controlled by a single dominant gene. A total 479 F₂:₃ lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to six publicly available and three recently developed wheat genomic SSR markers. The linked markers were localized to wheat chromosome 1B using Chinese Spring nulli-tetrasomic lines, and the resistance gene was localized to chromosome 1B based on SSR and wheat genomic information. A high-density genetic map was also produced. The pedigree, molecular marker data, and resistance response indicated that the stripe rust resistance gene in L693 is a novel gene, which was temporarily designated YrL693. The SSR markers that co-segregate with this gene (Xbarc187-1B, Xbarc187-1B-1, Xgwm18-1B, and Xgwm11-1B) have potential application in marker-assisted breeding of wheat, and YrL693 will be useful for broadening the genetic basis of stripe rust resistance in wheat.