Simultaneous saccharification and fermentation of cassava to succinic acid by Escherichia coli NZN111

• Published in: Bioresource technology Vol. 163; pp. 100 - 105
• Main Authors: Chen, Cuixia, Ding, Shaopeng, Wang, Dezheng, Li, Zhimin, Ye, Qin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2014; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; Carbohydrates - chemistry; Cassava; Density; Escherichia coli; Escherichia coli - metabolism; Escherichia coli NZN111; Fermentation; Fundamental and applied biological sciences. Psychology; Manihot - metabolism; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Productivity; Saccharification; Simultaneous saccharification and fermentation; Starches; Succinic acid; Succinic Acid - metabolism; Succinic acid; Escherichia coli NZN111; Simultaneous saccharification and fermentation; Cassava; Escherichia coli; Bacteria; Saccharification; Fermentation; Enterobacteriaceae
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.04.020

•Simultaneous saccharification and fermentation was applied to produce succinate from cassava.•Elevating the temperature to 40°C in anaerobic stage can improve the productivity efficiently.•The yield of succinate based on the amount of cassava starch achieved the highest 0.86g/g.•127.13 and 106.17g/L of succinate was obtained using cassava starch and powder, respectively. In this study, the production of succinic acid from cassava starch and raw cassava instead of glucose by Escherichia coli NZN111 was investigated. During the two-stage fermentation, simultaneous saccharification and fermentation (SSF) was applied in the anaerobic stage. The results showed that both the productivity and specific productivity in the process conducted at 40°C were higher than those in the cultivation conducted at 37°C. The yield of succinic acid based on the amount of added starch reached the highest level 0.86g/g and cassava starch was almost totally hydrolyzed in the SSF process. With the improved cell density, 127.13g/L of succinic acid was obtained. When the liquefied crude cassava powder was used directly in SSF, 106.17g/L of succinic acid was formed. The result showed that crude cassava powder could be another cheap raw material for succinic acid formation.