Changes of the reactor performance and the properties of granular sludge under tetracycline (TC) stress

• Published in: Bioresource technology Vol. 139; pp. 170 - 175
• Main Authors: Shi, Yijing, Xing, Sufang, Wang, Xinhua, Wang, Shuguang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2013; Elsevier
• Subjects: Applied sciences; Bacteria; Bacteria - drug effects; Biological and medical sciences; Biopolymers - analysis; Bioreactors - microbiology; EPS; Exact sciences and technology; Extracellular polymeric substances; Fluorescence; Fundamental and applied biological sciences. Psychology; Granular materials; Granular sludge; Granules; Inhibition; Microbial Sensitivity Tests; Oxidation; Particle Size; Pollution; Sewage - microbiology; Sludge properties; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Static Electricity; Stress, Physiological - drug effects; Tetracycline - chemistry; Tetracycline - pharmacology; Tetracycline stress; Tetracyclines; Time Factors; Wastes; Water Quality; Extracellular polymeric substances; Sludge properties; Granular sludge; Tetracycline stress; Polymer; Wastes; Reactor; Extracellular; Stress; Tetracycline
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.03.037

•TC has strong toxic effect on nitrifying granules and led to nitrite accumulation.•Sludge produced more EPS, with higher production of proteins than polysaccharides.•TC greatly changed protein functional groups but had little effect on polysaccharides.•The fluorescence of EPS was quenched by TC addition. In this study, the response of nitrifying granules (NG) and conventional granules (CG) to tetracycline (TC) was compared. The presence of TC made granules break down into small fractions and led to nitrite accumulation in nitrifying system; while it had little toxic effect on CG system. Specific oxygen uptake rate tests showed 3.3% inhibition of ammonium oxidizing bacteria and 25.7% inhibition of nitrite oxidizing bacteria in the NG system. Granules produced more extracellular polymeric substances (EPS) to protect themselves from the TC stress, with higher production of proteins compared to polysaccharides. FTIR data also revealed significant changes of protein functional groups, while only small changes in polysaccharides functional groups were found. Three dimensional excitation–emission matrix fluorescence spectroscopy showed a decrease of peak intensity, indicating quenching effect of TC on EPS fluorescence, and also a blue shift of peak position, indicating chemical changes of EPS components.