Heat shock factor 1 (HSF1) specifically potentiates c-MYC-mediated transcription independently of the canonical heat shock response
Despite its pivotal roles in biology, how the transcriptional activity of c-MYC is tuned quantitatively remains poorly defined. Here, we show that heat shock factor 1 (HSF1), the master transcriptional regulator of the heat shock response, acts as a prime modifier of the c-MYC-mediated transcription...
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Published in | Cell reports (Cambridge) Vol. 42; no. 6; p. 112557 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
27.06.2023
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Despite its pivotal roles in biology, how the transcriptional activity of c-MYC is tuned quantitatively remains poorly defined. Here, we show that heat shock factor 1 (HSF1), the master transcriptional regulator of the heat shock response, acts as a prime modifier of the c-MYC-mediated transcription. HSF1 deficiency diminishes c-MYC DNA binding and dampens its transcriptional activity genome wide. Mechanistically, c-MYC, MAX, and HSF1 assemble into a transcription factor complex on genomic DNAs, and surprisingly, the DNA binding of HSF1 is dispensable. Instead, HSF1 physically recruits the histone acetyltransferase general control nonderepressible 5 (GCN5), promoting histone acetylation and augmenting c-MYC transcriptional activity. Thus, we find that HSF1 specifically potentiates the c-MYC-mediated transcription, discrete from its canonical role in countering proteotoxic stress. Importantly, this mechanism of action engenders two distinct c-MYC activation states, primary and advanced, which may be important to accommodate diverse physiological and pathological conditions.
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•c-MYC and HSF1 comprise a transcription factor complex without heat stress•HSF1 physically couples c-MYC and GCN5 to promote histone acetylation•HSF1 association strengthens the genome-wide DNA binding of c-MYC•HSF1 augments the c-MYC-mediated transcriptional program
Xu et al. find that, under non-stressed conditions, HSF1, c-MYC, and MAX constitute a transcription factor complex, in sharp contrast with the assembly of HSF1 homotrimers during the heat shock response. HSF1 exerts a non-canonical transcriptional action in the absence of proteotoxic stress, amplifying c-MYC-mediated transcription genome wide. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 M.X., L.L., B.M.R., O.S., K.-H.C., S.D., K.-H.S., Z.T., and C.D. designed and conducted the experiments. C.D. conceptualized and supervised this study and analyzed the results. M.X. and C.D. wrote the manuscript. AUTHOR CONTRIBUTIONS |
ISSN: | 2211-1247 2211-1247 |
DOI: | 10.1016/j.celrep.2023.112557 |