Biodegradation of decabromodiphenyl ether (BDE-209) by a metal resistant strain, Bacillus cereus JP12

• Published in: Bioresource technology Vol. 149; pp. 8 - 15
• Main Authors: Lu, Mang, Zhang, Zhong-Zhi, Wu, Xue-Jiao, Xu, Yu-Xin, Su, Xiao-Li, Zhang, Min, Wang, Jing-Xiu
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.2013; Elsevier
• Subjects: Aerobic debromination; Aerobiosis - drug effects; Bacillus cereus; Bacillus cereus - drug effects; Bacillus cereus - growth & development; Bacillus cereus - metabolism; Bacteria; BDE-209; Biodegradation; Biodegradation, Environmental - drug effects; Biological and medical sciences; Biotechnology; Carbon; Ethers; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Halogenated Diphenyl Ethers - chemistry; Halogenated Diphenyl Ethers - metabolism; Halogenation - drug effects; Heavy metals; Metabolites; Metals, Heavy - toxicity; Spectroscopy, Fourier Transform Infrared; Strain; Surface chemistry; Surface-Active Agents - pharmacology; Time Factors; Metabolites; Aerobic debromination; Heavy metals; BDE-209; Biodegradation; Ether; Metabolite; Aerobe; Bacillaceae; Heavy metal; Bacillus cereus; Bacillales; Bacteria
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.09.040

•A new strain of aerobic bacterium, Bacillus cereus JP12, could effectively degrade BDE-209.•The first report on the degradation of BDE-209 by Bacillus cereus.•A slight stimulating effect on BDE-209 removal under low levels of Cu2+ and Zn2+.•JP12 could effectively degrade BDE-209 under high concentrations of Cu2+ and Zn2+.•Metabolite identification and deduction of a possible BDE-209 degradation pathway. A metal resistant bacterial strain, Bacillus cereus JP12, could use decabromodiphenyl ether (BDE-209) as the sole carbon and energy source for growth in mineral salt medium. Under the conditions of pH 6.0, 30°C, 150rpm and an inoculum of OD600=0.6, more than 88% of the initial BDE-209 (1mg/L) was degraded after 12days. The addition of appropriate surfactants and additional carbon sources could enhance the biodegradation efficiency of BDE-209. The presence of Cu2+ (⩽8mg/L) and Zn2+ (⩽15mg/L) provided a slight stimulating effect on BDE-209 removal. However, BDE-209 biodegradation efficiency was decreased when adding higher levels of metals due to reduced substrate availability caused by excess metal adsorption into the cell surface. Biosorption of heavy metals by JP12 led to release of light metals such as K+ and Na+. A BDE-209 biodegradation pathway was proposed on the basis of metabolite identification.