An anaerobic dynamic membrane bioreactor (AnDMBR) for landfill leachate treatment: Performance and microbial community identification

• Published in: Bioresource technology Vol. 161; pp. 29 - 39
• Main Authors: Xie, Zhenfang, Wang, Zhiwei, Wang, Qiaoying, Zhu, Chaowei, Wu, Zhichao
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.06.2014; Elsevier
• Subjects: Anaerobic biological treatment; Anaerobic treatment; Anaerobiosis; Applied sciences; Archaea; Bacteria; Biodiversity; Biofuels - analysis; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Bioreactors; Bioreactors - microbiology; Biotechnology; Communities; Dynamic membrane; Dynamics; Environment and pollution; Exact sciences and technology; Filtration; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Landfill leachate; Landfills; Membranes; Membranes, Artificial; Methane; Methanosarcina; Methods. Procedures. Technologies; Microbial Consortia; Pilot Projects; Pollution; Pyrosequencing; Various methods and equipments; Wastewater treatment; Wastewaters; Water Pollutants, Chemical - isolation & purification; Water Pollutants, Chemical - metabolism; Water Purification - instrumentation; Water treatment and pollution; Anaerobic biological treatment; Landfill leachate; Wastewater treatment; Pyrosequencing; Dynamic membrane; Chemiluminescence; Anaerobic digestion; Membrane reactor; Bioreactor; Sanitary landfill; Anaerobe; Membrane; Biological treatment; Waste water purification; Leachate; Sequencing; Microbial community
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.03.014

•Anaerobic dynamic membrane bioreactor was used for landfill leachate treatment.•62.2% of COD removal efficiency was achieved at the influent COD 13,000mg/L.•Average methane yield was 0.34L/g CODremoved at OLR 4.87kg COD/(m3d).•Archaeal community was stable while changes occurred in the bacterial community.•Genus Methanosarcina was identified as the dominant methanogen in the system. In this study, a pilot-scale anaerobic dynamic membrane bioreactor was operated for 142days for treating landfill leachate. Under stable operation, average COD removal efficiency of 62.2% was achieved when the reactor was fed with the raw leachate containing total ammonium concentration above 3000mg/L and COD above 13,000mg/L. The methane content in the biogas was in the range of 70–90%, and the average methane yield was 0.34L/g CODremoved at the organic loading rate of 4.87kg COD/(m3d). Pyrosequencing analyses indicated that during the operation the archaeal community was relatively stable while obvious changes took place in the bacterial community. Alkaliphilus, Petrimonas, Fastidiosipila and vadinBC27 were the abundant fermentation bacteria in bacterial communities. Moreover, phylum TM6 gradually became the most dominant bacterial community and reached the highest relative abundance of 32.9% as the operation elapsed. In archaeal communities, genus Methanosarcina was identified as the dominant methanogen.