Effectiveness of fluoride-containing toothpastes associated with different technologies to remineralize enamel after pH cycling: an in vitro study

• Published in: BMC oral health Vol. 22; no. 1; pp. 489 - 9
• Main Authors: Fernandes, Nayanna Lana Soares, Silva, José Gabriel Victor Costa, de Sousa, Elizabeth Barreto Galvão, D'Alpino, Paulo Henrique Perlatti, de Oliveira, Andressa Feitosa Bezerra, de Jong, Elbert de Josselin, Sampaio, Fábio Correia
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 14.11.2022; BioMed Central; BMC
• Subjects: Analysis; Animals; Biomimetics; Cariostatic Agents - therapeutic use; Cattle; Composition; Cross-Sectional Studies; Dental caries; Dental Caries - pathology; Dental Caries - prevention & control; Dental enamel; Dental Enamel - pathology; Dentifrices; Enamel; Fluorescence; Fluoride treatments; Fluorides; Fluorides - therapeutic use; Hardness; Health aspects; Humans; Hydrogen-Ion Concentration; Hydroxyapatite; Lesions; Light effects; Microscopy; Morphology; pH effects; Prevention; Risk factors; Scanning electron microscopy; Silica; Sodium Fluoride; Technology; Tooth remineralization; Tooth Remineralization - methods; Toothbrushing; Toothpaste; Toothpastes - therapeutic use; Brazil; Netherlands; Dental caries; Toothpaste; Biomimetics; Microscopy; Tooth remineralization; Hardness
• ISSN: 1472-6831; 1472-6831
• DOI: 10.1186/s12903-022-02429-2

To evaluate the efficacy of fluoride-containing toothpastes with different technologies to remineralize artificial caries lesions in enamel. Bovine enamel blocks were divided into three thirds: intact (untreated), demineralized (artificial caries lesion), and treated (caries lesion, pH cycling with dentifrices). Enamel blocks were randomly distributed into five groups (n = 12): Fluoride-free toothpaste, Colgate Oral Care (NC); Arginine-containing toothpaste, Colgate Total Daily Repair (PC); Silicate-based fluoride toothpaste: REFIX technology, regenerador + sensitive (RDC), NR-5 technology, Regenerate Enamel Science (RES), and NOVAMIN technology, Sensodyne Repair and Protect (SRP). The specimens were submitted to a pH cycling model for 6 days. The efficacy of the toothpastes was estimated by calculating the surface microhardness recovery (%SMH ) and the fluorescence recovery (ΔF ) with quantitative light-induced fluorescence. The cross-sectional micromorphology of the enamel surface was also assessed using scanning electron microscopy. Elemental analyses (weight%) were determined with an energy-dispersive X-ray spectrometer (EDS). The results were compared to that of the control (NC). Data were statistically analyzed (5%). %SMH could be ranked as follows: RDC = PC = RES = SRP > NC. Significantly higher %SMH and ΔF means were observed after enamel treatment with RDC (22.7 and 46.9, respectively). PC (%SMH  = 18.8) was as efficacious as RDC to recover the surface microhardness with a significantly lower mean of ΔF (19.5). Only RDC was able to promote the formation of a mineralized layer on the surface of enamel enriched with silicon on the surface. The silicate-based fluoride toothpaste containing REFIX technology demonstrated greater efficacy in the remineralizing artificial caries than the other products.