Engineered Bacteria as Drug Delivery Vehicles: Principles and Prospects

• Published in: Engineering Microbiology Vol. 2; no. 3; p. 100034
• Main Authors: Zhou, Yuxi, Han, Yong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2022; Elsevier
• Subjects: AI, autoinducer; AIP, autoinducing peptides; ANN, artificial neural network; aTc, anhydrotetracycline; CAP, capsular polysaccharide; Drug delivery; DSF, diffusible signal factor; Engineered bacteria; engineered bacteria Abbreviations AHL, acyl-homoserine lactone; FDA, U.S. Food and Drug Administration; FP, fusion peptides; GFP; GIT, gastrointestinal tract; GM, genetically modified; green fluorescent protein; HA1, hemagglutinin 1; HIV, human immunodeficiency virus; IPTG, isopropyl β-d-1-thiogalactopyranoside; LAB, lactic acid bacteria; MV, membrane vesicle; NICE, nisin-controlled expression; NRPS, non-ribosomal peptide synthase; OMV, outer membrane vesicle; PD-L1, programmed death-ligand 1; PEDV, porcine epidemic diarrhea virus; PKS, polyketide synthase; PQS, Pseudomonas quinolone signal; QS, quorum sensing; Review; RiPPs, ribosomal synthesized and post-translationally modified peptides; SARS, severe acute respiratory syndrome; SLIC, synchronized lysing integrated circuit; SP, signal peptide; STING, stimulator of interferon gene; SUSAR, suspected unexpected serious adverse reaction; Synthetic biology; RiPPs, ribosomal synthesized and post-translationally modified peptides; aTc, anhydrotetracycline; QS, quorum sensing; PD-L1, programmed death-ligand 1; AI, autoinducer; drug delivery; engineered bacteria Abbreviations AHL, acyl-homoserine lactone; FP, fusion peptides; PQS, Pseudomonas quinolone signal; GFP; PEDV, porcine epidemic diarrhea virus; PKS, polyketide synthase; GM, genetically modified; CAP, capsular polysaccharide; DSF, diffusible signal factor; FDA, U.S. Food and Drug Administration; SP, signal peptide; SUSAR, suspected unexpected serious adverse reaction; IPTG, isopropyl β-d-1-thiogalactopyranoside; HIV, human immunodeficiency virus; NICE, nisin-controlled expression; SARS, severe acute respiratory syndrome; OMV, outer membrane vesicle; SLIC, synchronized lysing integrated circuit; AIP, autoinducing peptides; GIT, gastrointestinal tract; LAB, lactic acid bacteria; green fluorescent protein; synthetic biology; STING, stimulator of interferon gene; ANN, artificial neural network; MV, membrane vesicle; NRPS, non-ribosomal peptide synthase; HA1, hemagglutinin 1; Engineered bacteria; Drug delivery; Synthetic biology
• ISSN: 2667-3703; 2097-4280; 2667-3703
• DOI: 10.1016/j.engmic.2022.100034

The development of drug delivery vehicles is in significant demand in the context of precision medicine. With the development of synthetic biology, the use of genetically engineered bacteria as drug delivery vectors has attracted more and more attention. Herein, we reviewed the research advances in bioengineered bacteria as drug carriers, with emphasis on the synthetic biology strategies for modifying these bacteria, including the targeted realization method of engineered bacteria, the designing scheme of genetic circuits, and the release pathways of therapeutic compounds. Based on this, the essential components, design principles, and health concerns of engineering bacteria as drug carriers and the development prospects in this field have been discussed. [Display omitted]