Peritransplant Monitoring of Immune Cell Function in Adult Living Donor Liver Transplantation

• Published in: Transplantation proceedings Vol. 42; no. 7; pp. 2567 - 2571
• Main Authors: Hwang, S, Kim, K.H, Song, G.W, Yu, Y.D, Park, G.C, Kim, K.W, Choi, N.K, Park, P.J, Choi, Y.I, Jung, D.H, Ahn, C.S, Moon, D.B, Ha, T.Y, Lee, S.G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.09.2010; Elsevier
• Subjects: Adenosine Triphosphate - metabolism; Adult; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hepatitis B - complications; Humans; Immunoassay - methods; Immunosuppressive Agents - therapeutic use; Liver Cirrhosis - surgery; Liver Cirrhosis - virology; Liver Diseases - classification; Liver Diseases - surgery; Liver Transplantation - immunology; Liver, biliary tract, pancreas, portal circulation, spleen; Living Donors; Lymphocyte Activation; Medical sciences; Middle Aged; Perioperative Period; Postoperative Period; Reference Values; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; T-Lymphocytes - immunology; T-Lymphocytes, Helper-Inducer - immunology; Tissue, organ and graft immunology; Human; Cell function; Digestive system; Living donor; Liver; Homotransplantation; Immunity; Medicine; Treatment; Surveillance; Surgery; Adult; Graft; Monitoring; Liver transplantation
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2010.04.040

Abstract Purpose We evaluated the clinical utility of peritransplant in vitro assays of immune cell function in adult living donor liver transplant (LDLT) recipients. Methods In particular, we measured immune cell function, using the ImmuKnow assay, in 107 adult LDLT recipients and 200 potential living liver donors (control group) admitted to our center between July 2008 and January 2009. Results In the control group, the mean proportion of T-helper/inducer cells was 36.8% ± 8.2%. The degree of immune response was strong in 12%, moderate in 77%, and low in 11%. In the study group, the degree of immune response within the first month was strong in 4.6%, moderate in 38.2%, and low in 57.2%, thus significantly lower than in the control group ( P < .001). ImmuKnow results and tacrolimus levels did not show a significant correlation ( r2 = .002, P = .392). Although six patients showed biopsy-proven acute cellular rejection, none showed a strong immune response. Patients with overt infection showed a lower immune response. Conclusions These results indicate that peritransplant assessment of immune response using the ImmuKnow assay does not reliably predict the occurrence of acute rejection. Additional studies are necessary to accurately assess the clinical utility of immune response monitoring.