Advances in the dynamic control of metabolic pathways in Saccharomyces cerevisiae

• Published in: Engineering Microbiology Vol. 3; no. 4; p. 100103
• Main Authors: Xiao, Chufan, Pan, Yuyang, Huang, Mingtao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2023; Elsevier
• Subjects: Dynamic control; Metabolic engineering; Response element; Review; Transcription regulation; Dynamic control; Metabolic engineering; Response element; Transcription regulation
• ISSN: 2667-3703; 2097-4280; 2667-3703
• DOI: 10.1016/j.engmic.2023.100103

The metabolic engineering of Saccharomyces cerevisiae has great potential for enhancing the production of high-value chemicals and recombinant proteins. Recent studies have demonstrated the effectiveness of dynamic regulation as a strategy for optimizing metabolic flux and improving production efficiency. In this review, we provide an overview of recent advancements in the dynamic regulation of S. cerevisiae metabolism. Here, we focused on the successful utilization of transcription factor (TF)-based biosensors within the dynamic regulatory network of S. cerevisiae. These biosensors are responsive to a wide range of endogenous and exogenous signals, including chemical inducers, light, temperature, cell density, intracellular metabolites, and stress. Additionally, we explored the potential of omics tools for the discovery of novel responsive promoters and their roles in fine-tuning metabolic networks. We also provide an outlook on the development trends in this field. [Display omitted]