Development of sample preparation method for isoliquiritigenin, liquiritin, and glycyrrhizic acid analysis in licorice by ionic liquids-ultrasound based extraction and high-performance liquid chromatography detection

• Published in: Food chemistry Vol. 138; no. 1; pp. 173 - 179
• Main Authors: Yang, Lei, Li, Li-li, Liu, Ting-ting, Zu, Yuan-gang, Yang, Feng-jian, Zhao, Chun-jian, Zhang, Lin, Chen, Xiao-qiang, Zhang, Zhong-hua
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.05.2013; Elsevier
• Subjects: analysis; anions; Biological and medical sciences; bromides; cations; Chalcones; Chalcones - analysis; Chalcones - isolation & purification; Chemical Fractionation; Chemical Fractionation - methods; chemistry; Chromatography, High Pressure Liquid; Flavanones; Flavanones - analysis; Flavanones - isolation & purification; Food engineering; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Glucosides; Glucosides - analysis; Glucosides - isolation & purification; Glycyrrhiza; Glycyrrhiza - chemistry; Glycyrrhiza glabra; Glycyrrhizic Acid; Glycyrrhizic Acid - analysis; Glycyrrhizic Acid - isolation & purification; glycyrrhizin; high performance liquid chromatography; Ionic liquid-based ultrasonic-assisted extraction; Ionic Liquids; Ionic Liquids - chemistry; isolation & purification; Isoliquiritigenin; Licorice; Liquiritin; methods; Methods of analysis, processing and quality control, regulation, standards; Plant Extracts; Plant Extracts - analysis; Plant Extracts - isolation & purification; soaking; Ultrasonics; Ultrasonics - methods; Isoliquiritigenin; Licorice; Glycyrrhizic acid; Liquiritin; Ionic liquid-based ultrasonic-assisted extraction; HPLC chromatography; Liquid; Analysis method; Sample preparation; Analysis; Development; Extraction; Control method; Detection; Ultrasound
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2012.10.059

► Licorice components were first extracted by ionic liquid based method. ► The efficiency in this work is higher than that of the conventional solvent method. ► The proposed approach was confirmed by good recovery and reproducibility. ► The proposed approach has eco-friendly, convenient, high practical value technique. An ionic liquid-based ultrasonic-assisted extraction (ILUAE) method had been used for the effective extraction of isoliquiritigenin (IQ), liquiritin (LQ) and glycyrrhizic acid (GA) from licorice. The ionic liquids with different cations and anions were investigated in this work and 0.5M 1-butyl-3-methylimidazolium bromide solution was selected as solvent. In addition, the technical parameters including soaking time, solid–liquid ratio, ultrasonic power and time were optimized. Compared with the conventional solvent extraction, the proposed approach exhibited higher efficiency, which indicated the ILUAE was an efficient, rapid and simple sample preparation technique. There was no degradation of the target analytes had been observed at the optimum conditions which was evidenced by the stability studies performed with standard of IQ, LQ and GA. The proposed method also showed high reproducibility and was environmental friendly.