Genetic linkage maps of two yellowtails ( Seriola quinqueradiata and Seriola lalandi)

• Published in: Aquaculture Vol. 244; no. 1; pp. 41 - 48
• Main Authors: Ohara, Eriko, Nishimura, Takuya, Nagakura, Yoshitomo, Sakamoto, Takashi, Mushiake, Keiichi, Okamoto, Nobuaki
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 28.02.2005; Elsevier Science; Elsevier Sequoia S.A
• Subjects: Animal aquaculture; Animal productions; Aquaculture; Biological and medical sciences; chromosome mapping; fish culture; Fisheries; Fundamental and applied biological sciences. Psychology; General aspects; Genetic linkage map; genetic variation; Genetics; linkage (genetics); mariculture; Marine; marine fish; Microsatellite; microsatellite repeats; Seriola; Seriola lalandi; Seriola quinqueradiata; Yellowtail; INW, Japan; Yellowtail; Microsatellite; Seriola quinqueradiata; Seriola lalandi; Genetic linkage map; Marine environment; Vertebrata; Pisces; Genetic linkage; Aquaculture
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2004.10.022

The yellowtails Seriola quinqueradiata and Seriola lalandi are the most important species in marine fishery resources and aquaculture in Japan. A genetic linkage map is needed to improve efficiency of breeding by marker-assisted selection (MAS) and for the identification of commercially important genes. Therefore, we have constructed a genetic linkage map for the yellowtails using microsatellite markers. Microsatellites were isolated from a genomic DNA library of S. quinqueradiata. Segregation of 217 microsatellites was studied in 90 progeny from a cross between S. quinqueradiata and S. lalandi. These were used to construct separate linkage maps of a female ( S. quinqueradiata) and a male ( S. lalandi). Twenty-five linkage groups were distinguished in the female ( S. quinqueradiata) map, which spanned for 473.3 centiMorgans (cM) with an average intermarker distance of 2.7 cM (total length estimated: 901.7 cM), and 21 linkage groups were distinguished in the male ( S. lalandi) map, which spanned 584.3 cM with an average intermarker distance of 4.8 cM (total length estimated: 1715.3 cM). The microsatellite loci and genetic linkage maps will increase the efficiency of selective breeding programs for yellowtails.