Metagenomic sequencing of human cardiac tissue reveals Microbial RNA which correlates with Toll-like receptor-associated inflammation in patients with heart disease

• Published in: Scientific reports Vol. 13; no. 1; p. 7884
• Main Authors: Sandstedt, Joakim, Vukusic, Kristina, Dellgren, Göran, Jeppsson, Anders, Mattsson Hultén, Lillemor, Rotter Sopasakis, Victoria
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.05.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/208; 631/337; 631/553; 692/4017; 692/4019; Aortic valve; Atrial Fibrillation; Cardiovascular disease; Cardiovascular diseases; Cell growth; Cell interactions; Clinical Medicine; Coronary artery disease; gene; gut microbiota; Heart Diseases; Heart surgery; Heart Valve Prosthesis Implantation; Humanities and Social Sciences; Humans; Inflammation; Inflammation - genetics; injury; kefiran; Klinisk medicin; link; Metagenomics; multidisciplinary; myocardial-infarction; Myocardium; Organ donors; pathogen burden; Rheumatic heart disease; Ribonucleic acid; risk; RNA; Science; Science & Technology - Other Topics; Science (multidisciplinary); survival; Toll-like receptors; Toll-Like Receptors - genetics; Treatment Outcome
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-35157-w

Cardiovascular disease (CVD) is strongly associated with chronic low-grade inflammation, involving activated Toll-like receptors and their downstream cellular machinery. Moreover, CVD and other related inflammatory conditions are associated with infiltration of bacteria and viruses originating from distant body sites. Thus, in this study we aimed to map the presence of microbes in the myocardium of patients with heart disease that we previously found to display upregulated Toll-like receptor signaling. We performed metagenomics analysis of atrial cardiac tissue from patients undergoing coronary artery bypass grafting (CABG) or aortic valve replacement (AVR) and compared with atrial cardiac tissue from organ donors. A total of 119 species of bacteria and seven species of virus were detected in the cardiac tissue. RNA expression of five bacterial species were increased in the patient group of which L . kefiranofaciens correlated positively with cardiac Toll-like receptor-associated inflammation. Interaction network analysis revealed four main gene set clusters involving cell growth and proliferation, Notch signaling, G protein signaling and cell communication in association with L. kefiranofaciens RNA expression. Taken together, intracardial expression of L . kefiranofaciens RNA correlates with pro-inflammatory markers in the diseased cardiac atrium and may have an effect on specific signaling processes important for cell growth, proliferation and cell communication.