Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite

• Published in: Advanced science Vol. 10; no. 13; pp. e2300038 - n/a
• Main Authors: Tong, Lei, Pu, Xiaocong, Liu, Quanying, Li, Xing, Chen, Manyu, Wang, Peilei, Zou, Yaping, Lu, Gonggong, Liang, Jie, Fan, Yujiang, Zhang, Xingdong, Sun, Yong
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.05.2023; John Wiley and Sons Inc; Wiley
• Subjects: 3D printing; Animals; Bone Regeneration; Bones; Collagen; Design; Ethanol; hydrogels; Hydroxyapatite; Mice; Mice, Nude; nanohydroxyapatite; Nanoparticles; Particle size; photoinitiation; Printing, Three-Dimensional; Prospective Studies; Rabbits; Scanning electron microscopy; Spectrum analysis; Stem cells; Tensile strength; Tissue Scaffolds - chemistry; Transplants & implants; Viscosity; nanohydroxyapatite; bone regeneration; hydrogels; 3D printing; photoinitiation
• ISSN: 2198-3844; 2198-3844
• DOI: 10.1002/advs.202300038

Nanostructured biomaterials that replicate natural bone architecture are expected to facilitate bone regeneration. Here, nanohydroxyapatite (nHAp) with vinyl surface modification is acquired by silicon‐based coupling agent and photointegrated with methacrylic anhydride‐modified gelatin to manufacture a chemically integrated 3D‐printed hybrid bone scaffold (75.6 wt% solid content). This nanostructured procedure significantly increases its storage modulus by 19.43‐fold (79.2 kPa) to construct a more stable mechanical structure. Furthermore, biofunctional hydrogel with biomimetic extracellular matrix is anchored onto the filament of 3D‐printed hybrid scaffold (HGel‐g‐nHAp) by polyphenol‐mediated multiple chemical reactions, which contributes to initiate early osteogenesis and angiogenesis by recruiting endogenous stem cells in situ. Significant ectopic mineral deposition is also observed in subcutaneously implanted nude mice with storage modulus enhancement of 25.3‐fold after 30 days. Meanwhile, HGel‐g‐nHAp realizes substantial bone reconstruction in the rabbit cranial defect model, achieving 61.3% breaking load strength and 73.1% bone volume fractions in comparison to natural cranium 15 weeks after implantation. This optical integration strategy of vinyl modified nHAp provides a prospective structural design for regenerative 3D‐printed bone scaffold. The photointegration of vinyl‐modified nanohydroxyapatite and gelatin significantly increases the storage modulus of 3D‐printed scaffold in comparison with traditional hydroxyapatite as bioink, and the anchored biomimetic extracellular matrix on the surface of filament based on polyphenol‐mediated multiple reactions synergistically enhances the substantial bone regeneration by availably initiating endogenous stem cell recruitment and osteogenic/angiogenic differentiation.