Research on the circular RNA bioinformatics in patients with acute myocardial infarction

• Published in: Journal of clinical laboratory analysis Vol. 35; no. 2; pp. e23621 - n/a
• Main Authors: Yin, Lianli, Tang, Yinghua, Jiang, Minghe
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.02.2021; John Wiley and Sons Inc
• Subjects: acute myocardial infarction; Apoptosis; Bioinformatics; Blood pressure; Cardiovascular disease; Chromatin; circRNA; Circular RNA; Coronary artery disease; Diabetes; Disease; DNA microarrays; Electrocardiography; Extracellular matrix; Gene expression; Genes; Heart attacks; Heart diseases; Heart failure; Hypertension; Kinases; MAP kinase; MicroRNAs; miRNA; Myocardial infarction; Patients; Peripheral blood; Signal transduction; Software; United States > US; China; circRNA; acute myocardial infarction; bioinformatics
• ISSN: 0887-8013; 1098-2825; 1098-2825
• DOI: 10.1002/jcla.23621

Objective Through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship and possible mechanisms with AMI. Method The study subjects included 3 AMI patients and 3 controls, and circRNA expression profiling analysis was performed using a microarray gene chip to identify circRNAs with large differences in expression between groups and to construct a circRNA‐miRNA network. Results Compared with the control group, there were 650 differentially expressed circRNAs found in AMI patients (P < .05, fold change > 2), including 535 up‐regulated circRNAs, such as hsa_circ_0050908, hsa_circRNA4010‐22, hsa_circ_0081241, hsa_circ_0010551, hsa_circRNA4010‐20, hsa_circRNA14702, hsa_circ_0115392, has_circRNA1825‐44, has_circRNA8493‐7, and hsa_circ_0025097. Furthermore, there were 115 down‐regulated circRNAs, such as hsa_circ_0066439, hsa_circ_0054211, hsa_circ_0095920, hsa_circ_0122984, hsa_circ_0113067, hsa_circ_0039155, hsa_circRNA4014‐45, hsa_circ_0122979, hsa_circ_0059665, and hsa_circ_0009319. The circRNAs hsa_circ_0066439, hsa_circ_0081241, and hsa_circ_0122984 can regulate multiple signal pathways to participate in the AMI process through hsa‐miR‐1254, hsa‐miR‐328‐5p, and other miRNAs. In addition, the expression of circRNA‐miRNA in peripheral blood is related to the network. Differentially expressed circRNAs are involved in chromatin organization, chromatin‐modifying enzymes, signal transduction, lysine degradation, the mitogen‐activated protein kinase (MAPK) signaling pathway, focal adhesion, and a variety of other pathways, such as myocardial infarction, coronary heart disease, hypertension, and other diseases. The gene ontology analysis results show that molecular function mainly involves binding and molecular structural activity, whereas the biological process mainly involves a single biological process, a cellular component for organization, and a cellular process, and the cellular component mainly involves a protein complex, an extracellular matrix, and a membrane. Conclusion circRNA and microRNA interact to participate in the development of AMI. circRNA may be involved in the pathogenesis of AMI. 650 circRNAs are differentially expressed in AMI disease, and the interaction between circRNA and miRNA is involved in the occurrence and development of AMI. Among them, hsa_circ_0066439, hsa_circ_0081241, hsa_circ_0122984, and other highly different circRNAs (hsa_circ_0043563, hsa_circ_0119137, hsa_circ_0025097, hsa_circ_0106804, hsa_circ_0085214, and hsa_circ_0028302) may regulate multiple signaling pathways by combining with miR‐328‐5p, and then participate in the pathogenesis of AMI, providing a new breakthrough point for AMI diagnosis or targeted therapy.