comparison of two column classification systems during the chromatographic analysis of steroids

• Published in: Journal of separation science Vol. 34; no. 23; pp. 3310 - 3321
• Main Authors: Plenis, Alina, Balakowska, Edyta, Bączek, Tomasz
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag 01.12.2011; WILEY-VCH Verlag; Wiley; Wiley Subscription Services, Inc
• Subjects: analysis; Analytical chemistry; Availability; Biological and medical sciences; chemical structure; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography, High Pressure Liquid; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Chromatography, Reverse-Phase; Chromatography, Reverse-Phase - instrumentation; Chromatography, Reverse-Phase - methods; Classification; Column classification; Columns (structural); Exact sciences and technology; instrumentation; Investigative techniques, diagnostic techniques (general aspects); liquid chromatography; Liquid chromatography (LC) separations; Liquids; Mathematical analysis; Medical sciences; methods; Miscellaneous. Technology; Other chromatographic methods; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; physicochemical properties; principal component analysis; Principal component analysis (PCA); Quantitative structure-retention relationships (QSRR); Retention; Steroid hormones; Steroids; Steroids - analysis; Urine; Biological fluid; Chemical analysis; Chromatographic retention; HPLC chromatography; Principal component analysis (PCA); Liquid chromatography (LC) separations; Clinical biology; Classification; Stationary phase; Human; Urine; Healthy subject; Steroid hormone; Prediction; Endogenous substance; Column classification; Reversed phase chromatography; Ultraviolet detector; Property structure relationship; Chemometrics; Comparative study; Bonded stationary phase; Quantitative structure-retention relationships (QSRR); Steroid hormones; Principal component analysis
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201100631

Nowadays, due to the availability of hundreds of brands of reversed‐phase liquid chromatographic columns, the selection of suitable columns can be difficult. Therefore, a good characterization and classification system is very important. Among published papers, the classification system based on quantitative structure–retention relationships and a method developed at the Katholieke Universiteit Leuven also exist. In quantitative structure–retention relationships, retention is evaluated in terms of the chemical structure of the analytes and the physicochemical properties of both the stationary and mobile phase. The second system allows to rank columns due to the values of four parameters and the calculation of specific FKUL‐values for a reference column and to be compared with others. In this paper, the classification systems based both on quantitative structure–retention relationships and the FKUL‐values using principal components analysis were compared. Moreover, the proposed column ranking systems have been checked in clinical practice case considering liquid chromatography determination of six steroid hormones in urine samples. Despite that the matching of both methods is not exactly the same, both classification systems provide simple, reliable and comparable results.