Overexpression of long non-coding RNA00355 enhances proliferation, chemotaxis, and metastasis in colon cancer via promoting GTF2B-mediated ITGA2

• Published in: Translational oncology Vol. 14; no. 1; p. 100947
• Main Authors: Ruan, Zhiyan, Deng, Hongling, Liang, Minhua, Xu, Zhe, Lai, Manxiang, Ren, Hong, Deng, Xiangliang, Su, Xinguo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2021; Neoplasia Press; Elsevier
• Subjects: Colon cancer; General transcription factor II-B; Integrin alpha-2; Invasion; Long non-coding RNA00355; Migration; Original Research; General transcription factor II-B; Integrin alpha-2; Colon cancer; Migration; Invasion; Long non-coding RNA00355
• ISSN: 1936-5233; 1936-5233
• DOI: 10.1016/j.tranon.2020.100947

•LncRNA LINC00355 promotes colon cancer malignancy.•LncRNA LINC00355 positively regulates ITGA2 via recruiting GTF2B.•LncRNA LINC00355 positively regulates GTF2B-mediated ITGA2 to promote colon cancer.•This study proposes a novel targeted strategy for cancer treatment. Long non-coding RNAs (LncRNAs) can regulate physiological and pathological functions, exhibiting a wide range of roles in cell biology. Moreover, many lncRNAs are dysregulated in various cancers, including colon cancer. In this study, we investigated the role of the lncRNA LINC00355 in colon cancer, after first establishing its interaction with GTF2B, and ITGA2 on the LncMap database. The predicted relationships between the lncRNA LINC00355, GTF2B, and ITGA2 were identified using luciferase reporter assay, RIP, and ChIP experiments. Western blot analysis and RT-qPCR were applied to determine expression pattern of lncRNA LINC00355 and ITGA2 in colon cancer cells. Additionally, EdU, TUNEL, Cell-adhesion and Transwell assay was used for the detection of the effects of this axis on proliferation, apoptosis, adhesion, chemotaxis and metastasis. LncRNA LINC00355 targeted IGFBP2 through the recruitment of GTF2B. LncRNA LINC00355 was highly expressed in colon cancer cells, and overexpression of lncRNA LINC00355 increased the expression of IGFBP2 and GTF2B, and thereby promoted the proliferation, chemotaxis, invasion, and migration in colon cancer. In summary, downregulation of lncRNA LINC00355 in colon cancer inhibited tumor growth in colon cancer through effects on the GTF2B/IGFBP2 axis.