The Mechanical Power of Titin Folding

• Published in: Cell reports (Cambridge) Vol. 27; no. 6; pp. 1836 - 1847.e4
• Main Authors: Eckels, Edward C., Haldar, Shubhasis, Tapia-Rojo, Rafael, Rivas-Pardo, Jaime Andrés, Fernández, Julio M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.05.2019; Elsevier
• Subjects: Biomechanical Phenomena; Connectin - chemistry; Connectin - metabolism; disulfide bond; Disulfides - metabolism; force spectroscopy; magnetic tweezers; mechanical power; Models, Biological; Molecular Chaperones - metabolism; muscle contraction; Oxidation-Reduction; oxidative folding; oxidoreductase; Oxidoreductases - metabolism; Peptides - metabolism; Protein Disulfide-Isomerases - metabolism; Protein Domains; Protein Folding; single molecule; titin; titin; single molecule; muscle contraction; disulfide bond; oxidative folding; magnetic tweezers; mechanical power; force spectroscopy; protein folding; oxidoreductase
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2019.04.046

The delivery of mechanical power, a crucial component of animal motion, is constrained by the universal compromise between the force and the velocity of its constituent molecular systems. While the mechanisms of force generation have been studied at the single molecular motor level, there is little understanding of the magnitude of power that can be generated by folding proteins. Here, we use single-molecule force spectroscopy techniques to measure the force-velocity relation of folding titin domains that contain single internal disulfide bonds, a common feature throughout the titin I-band. We find that formation of the disulfide regulates the peak power output of protein folding in an all-or-none manner, providing at 6.0 pN, for example, a boost from 0 to 6,000 zW upon oxidation. This mechanism of power generation from protein folding is of great importance for muscle, where titin domains may unfold and refold with each extension and contraction of the sarcomere. [Display omitted] •Real-time observation of disulfide reduction and oxidation using magnetic tweezers•Oxidation of titin Ig domains shifts the midpoint folding force from 4.1 to 12.5 pN•An 8-repeat oxidized titin construct reaches folding speeds of up to 1,900 nm/s•Peak power output of the same oxidized titin construct is 6,000 zW Eckels et al. use single-molecule magnetic tweezers to simultaneously probe the folding dynamics of titin Ig domains and monitor the redox status of single disulfides within the Ig fold. Oxidation of the disulfide bond greatly increases both the folding force and the magnitude of power delivered by protein folding.