Lung carcinomas do not induce T-cell apoptosis via the Fas/Fas ligand pathway but down-regulate CD3 epsilon expression

• Published in: Cancer Immunology, Immunotherapy Vol. 57; no. 3; pp. 325 - 336
• Main Authors: Prado-Garcia, Heriberto, Aguilar-Cazares, Dolores, Meneses-Flores, Manuel, Morales-Fuentes, Jorge, Lopez-Gonzalez, Jose Sullivan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.03.2008; Springer; Springer Nature B.V
• Subjects: Adult; Aged; Antineoplastic agents; Apoptosis; Apoptosis - immunology; Biological and medical sciences; Cancer Research; Carcinoma, Non-Small-Cell Lung - immunology; CD28 Antigens - genetics; CD28 Antigens - metabolism; CD3 Complex - genetics; CD3 Complex - metabolism; Cell Line, Tumor; Cells; Coculture Techniques; Down-Regulation - genetics; Down-Regulation - immunology; Fas Ligand Protein - analysis; Fas Ligand Protein - biosynthesis; Flow Cytometry; Humans; Hypotheses; Immune system; Immunohistochemistry; Immunology; Immunotherapy; Jurkat Cells; Ligands; Lung cancer; Lung Neoplasms - immunology; Lymphocytes; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original; Original Article; Pharmacology. Drug treatments; Pneumology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; RNA, Messenger - immunology; Signal Transduction - immunology; T-Lymphocytes - immunology; Tumors of the respiratory system and mediastinum; Mexico; CD3 zeta chain; Non-small cell lung carcinoma; Tumor counterattack; Tumor-derived microvesicles; CD3 epsilon chain; Fas Ligand; Lung disease; Fas ligand; Respiratory disease; Lung cancer; Cytokine; CD3 epsilon chain,CD3 zeta chain; Malignant tumor; Bronchopulmonary carcinoma; non-small cell lung carcinoma; Gene expression; Bronchopulmonary; Microvesicle; Treatment; Tumor necrosis factor; Cell death; Immunotherapy; T-Lymphocyte; Bronchus disease; Cancer; Apoptosis
• ISSN: 0340-7004; 1432-0851
• DOI: 10.1007/s00262-007-0372-6

Background Non-small cell lung carcinoma (NSCLC) patients have impaired cellular immune responses. It has been hypothesized that tumor cells expressing Fas Ligand (FasL) induce in T lymphocytes: (a) apoptosis (tumor counterattack) and (b) down-regulation of CD3ζ expression. However, the hypothesis of tumor counterattack is still controversial. Methods We analyzed FasL expression on NSCLC cell lines and on tumor cells from lung adenocarcinoma patients by flow cytometry and immunocytochemistry. FasL mRNA expression was detected in NSCLC cell lines using RT-PCR, and functional FasL was evaluated on Fas-expressing Jurkat T-cells by annexin-V-FITC staining and by SubG 1 peak detection. Also, the proapoptotic effect of microvesicles released from NSCLC cell lines in Jurkat T-cells was studied. Alterations in the expression levels of CD3ζ, CD3ε, and CD28 [measured as mean fluorescence intensity (MFI)] were determined in Jurkat T-cells after co-culture with NSCLC cell lines or tumor-derived microvesicles. Furthermore, the expression levels of CD3ζ and CD3ε in CD4+T and CD8+T lymphocytes from lung adenocarcinoma patients was studied. Results Our results indicate that NSCLC cells neither FasL expressed nor induced apoptosis in Jurkat T-cells. Tumor-derived microvesicles did not induce apoptosis in Jurkat T-cells. In contrast, NSCLC cell lines down-regulated CD3ε but not CD3ζ chain expression in Jurkat T-cells; this effect was induced by soluble factors but not by microvesicles. In lung adenocarcinoma patients, significant decreases of MFI values for CD3ε, but not CD3ζ, were found in CD4+T and CD8+T cells from pleural effusion compared to peripheral blood and in peripheral blood of patients compared to healthy donors. Conclusions Our data do not support the tumor counterattack hypothesis for NSCLC. Nonetheless, down-regulation of CD3ε in T-cells induced by NSCLC cells might lead to T-cell dysfunction.