The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action

• Published in: Food chemistry. Molecular sciences Vol. 4; p. 100093
• Main Authors: Lazarini, Josy Goldoni, Massarioli, Adna Prado, Soares, Jackeline Cintra, Nani, Bruno Dias, Charo, Nancy, Oliveira, Douglas Souza, Camargo, Lauren, Alvarez-Flores, Miryam Paola, Batista, Isabel de Fátima Correia, Chudzinski-Tavassi, Ana Marisa, Alencar, Severino Matias de, Franchin, Marcelo, Rosalen, Pedro Luiz
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30.07.2022; Elsevier
• Subjects: Anti-inflammatory; Eugenia selloi; Native fruit; ROS and RNS; Eugenia selloi; Native fruit; Anti-inflammatory; ROS and RNS
• ISSN: 2666-5662; 2666-5662
• DOI: 10.1016/j.fochms.2022.100093

•Purified subfraction from Eugenia selloi fruit, showed anti-inflammatory activity.•It were identified isomers of quercetrin, vanillic acid, and coumaric acid.•S8 reduced NF-κB, IL-1β, IL-6, IL-10, MDC and MCP-1 levels in macrophages.•S8 reduced neutrophil migration and ICAM-1 expression in mice.•S8 showed scavenging capacity against ROO, HOCl and NO biological radicals. We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.