Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I – Thymidine kinase/ganciclovir in glioma

• Published in: Cancer letters Vol. 297; no. 1; pp. 84 - 90
• Main Authors: Jang, Su Jin, Kang, Joo Hyun, Kim, Kwang Il, Lee, Tae Sup, Lee, Yong Jin, Lee, Kyo Chul, Woo, Kwang Sun, Chung, Wee Sup, Kwon, Hee Chung, Ryu, Chun Jeih, Choi, Tae Hyun, Choi, Chang Woon, Lim, Sang Moo, Cheon, Gi Jeong
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.11.2010; Elsevier Limited
• Subjects: Animals; Antineoplastic Agents - pharmacology; Bioluminescence; Brain Neoplasms - enzymology; Brain Neoplasms - genetics; Brain Neoplasms - therapy; Cell Line, Tumor; Cell Survival - drug effects; Deoxyribonucleic acid; DNA; Dose-Response Relationship, Drug; Enzymes; Female; Ganciclovir - metabolism; Ganciclovir - pharmacology; Gene expression; Gene therapy; Genes, Reporter; Genes, Transgenic, Suicide; Genetic Therapy - methods; Genetic Vectors - genetics; Glioblastoma - enzymology; Glioblastoma - genetics; Glioblastoma - therapy; Glioma; Hematology, Oncology and Palliative Medicine; Herpes simplex virus; Herpesvirus 1, Human - enzymology; Herpesvirus 1, Human - genetics; HSV1-tk; Idoxuridine - analogs & derivatives; Idoxuridine - metabolism; Kinases; Kinetics; Lentivirus - genetics; Luciferase; Luciferases, Firefly - biosynthesis; Luciferases, Firefly - genetics; Luminescent Measurements; Medicine; Mice; Mice, Inbred BALB C; Mice, Nude; Molecular imaging; Nuclear medicine; Positron-Emission Tomography; Proteins; Rats; Recombinant Fusion Proteins - metabolism; Suicidal gene therapy; Thymidine Kinase - biosynthesis; Thymidine Kinase - genetics; Tumor Burden - drug effects; Molecular imaging; Suicidal gene therapy; HSV1-tk; Glioma; Luciferase
• ISSN: 0304-3835; 1872-7980; 1872-7980
• DOI: 10.1016/j.canlet.2010.04.028

Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [ 125I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [ 18F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement.