Short- and long-term haematological surveillance of healthy donors of allogeneic peripheral haematopoietic progenitors mobilized with G-CSF: a single institution prospective study

• Published in: Bone marrow transplantation (Basingstoke) Vol. 36; no. 4; pp. 289 - 294
• Main Authors: TASSI, C, TAZZARI, P. L, BONIFAZI, F, GIUDICE, V, NANNETTI, A, RICCI, F, RIZZI, S, BANDINI, G, CONTE, R
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 01.08.2005
• Subjects: Adult; Age Factors; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Apheresis; Biological and medical sciences; Blood; Blood Cell Count; Blood platelets; Bone marrow; Bone marrow, stem cells transplantation. Graft versus host reaction; Care and treatment; Correlation; Female; Follow-Up Studies; Granulocyte colony-stimulating factor; Granulocyte Colony-Stimulating Factor - administration & dosage; Granulocyte Colony-Stimulating Factor - pharmacology; Health aspects; Hematology; Hematopoietic Stem Cell Mobilization - methods; Hematopoietic stem cells; Humans; Leukapheresis; Leukemia; Leukocytes (neutrophilic); Lymphocyte Count; Lymphocytes; Lymphopenia; Lymphopenia - etiology; Male; Medical sciences; Middle Aged; Neutrophils - cytology; Organ donors; Peripheral blood; Peripheral Blood Stem Cell Transplantation; Physiological aspects; Platelet Count; Platelets; Population Surveillance; Prospective Studies; Stem cell transplantation; Surveillance; Time Factors; Tissue Donors; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation; Transplantation, Homologous; Italy; Short term; Hematology; Stem cell; Hematopoietic cell; Homograft; G-CSF adverse side effects; Long term; G-CSF; allogeneic healthy donors; Prospective; Mobilization; Granulocyte colony stimulating factor; Donor; Surveillance; Secondary effect; Graft
• ISSN: 0268-3369; 1476-5365
• DOI: 10.1038/sj.bmt.1705066

Healthy allogeneic donors, who were treated with G-CSF and underwent peripheral blood haematopoietic precursor collection at our Institution, were enrolled in a short- and long-term haematological surveillance protocol for a 5--7--year period. To date, 94 donors have been assessed with a mean follow-up of 30 months (4--84); for 30 subjects, the follow-up is >or=48 months. During G-CSF administration, 23/94 donors showed a significant platelet count decrease from the baseline. Pre-apheresis platelet decrement correlated with the total G-CSF dose administered, baseline platelet level and donor age. Normal platelet counts returned within 4--8 months. PMN and/or lymphocyte lower values were observed in 55/94 donors 2 weeks after G-CSF administration, with mean drops from the baseline of 40 and 36% for PMN and lymphocytes, respectively. The PMN decrease correlated inversely with donor age, as younger donors were more affected than older ones, whereas the lymphocyte decrease correlated directly with the total blood volumes processed in the apheresis courses, in particular for donors subjected to large volume leukaphereses. Long-term observation showed moderate neutrophil reduction (25% count drop from the baseline) in four of the 30 donors observed for four years or more. 14 donors showed persistent, slight lymphocytopenia (mean drop of 13%) until the third year, with recovery in the fourth year of follow-up.