Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle

• Published in: Stem cell reports Vol. 2; no. 1; pp. 92 - 106
• Main Authors: Castiglioni, Alessandra, Hettmer, Simone, Lynes, Matthew D., Rao, Tata Nageswara, Tchessalova, Daria, Sinha, Indranil, Lee, Bernard T., Tseng, Yu-Hua, Wagers, Amy J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.01.2014; Elsevier
• Subjects: Adipogenesis; Animals; Antigens, CD - metabolism; Cell Differentiation; Cell Lineage; Cells, Cultured; Fetus - cytology; Flow Cytometry; Humans; Integrin alpha Chains - metabolism; Mice; Muscle Development; Muscle, Skeletal - cytology; Osteogenesis; PAX7 Transcription Factor - metabolism; Resource; Stem Cell Transplantation; Stem Cells - cytology; Stem Cells - metabolism; Transplantation, Heterologous
• ISSN: 2213-6711; 2213-6711
• DOI: 10.1016/j.stemcr.2013.12.006

Fluorescence-activated cell sorting (FACS) strategies to purify distinct cell types from the pool of fetal human myofiber-associated (hMFA) cells were developed. We demonstrate that cells expressing the satellite cell marker PAX7 are highly enriched within the subset of CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi hMFA cells. These CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi cells lack adipogenic capacity but exhibit robust, bipotent myogenic and osteogenic activity in vitro and engraft myofibers when transplanted into mouse muscle. In contrast, CD45−CD11b−GlyA−CD31−CD34+ fetal hMFA cells represent stromal constituents of muscle that do not express PAX7, lack myogenic function, and exhibit adipogenic and osteogenic capacity in vitro. Adult muscle likewise contains PAX7+ CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi hMFA cells with in vitro myogenic and osteogenic activity, although these cells are present at lower frequency in comparison to their fetal counterparts. The ability to directly isolate functionally distinct progenitor cells from human muscle will enable novel insights into muscle lineage specification and homeostasis. •CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi human fetal muscle cells are PAX7+•CD34−CD56intITGA7hi fetal cells have bipotent myogenic/osteogenic activity in vitro•PAX7+ CD34−CD56intITGA7hi cells are fewer in number in adult versus fetal muscle This work by Castiglioni et al. establishes the presence of functionally distinct cell populations within the pool of myofiber-associated cells in human fetal and adult skeletal muscle and reports effective methods for the direct isolation by fluorescence-activated cell sorting of a cell population in fetal muscle with bipotent in vitro myogenic/osteogenic activity.