Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)

• Published in: Analytical and bioanalytical chemistry Vol. 397; no. 3; pp. 1389 - 1393
• Main Authors: Granado-Lorencio, F, Herrero-Barbudo, C, Blanco-Navarro, I, Pérez-Sacristán, B
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.06.2010; Springer-Verlag; Springer
• Subjects: 25-OH-vitamin D; Alfacalcidol; Analysis; Analytical Chemistry; Biochemistry; Biological markers; Calcifediol; Carotenes; Carotenoids; Carotenoids - blood; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Comparative analysis; Exact sciences and technology; Food Science; High performance liquid chromatography; Humans; Laboratory Medicine; Linear Models; Liquid chromatography; Lycopene; Mathematical analysis; Methods; Monitoring/Environmental Analysis; Nutrition; Nutritional aspects; nutritional status; Other chromatographic methods; Physiological aspects; Product/Service Evaluations; Sensitivity and Specificity; Serum; Spectrometric and optical methods; Technical Note; Tocopherol; Tocopherols; UHPLC; Vitamin A; Vitamin A - blood; Vitamin D; Vitamin D - blood; Vitamin E - blood; Vitamins; Vitamins - blood; Spain; Carotenoids; UHPLC; Tocopherol; Nutritional status; 25-OH-vitamin D; Retinol; Vitamin; HPLC chromatography; Biological marker; Ester; Accuracy; Serum; Human; Elution; Gradient; Carotene; Sample; Use; Method; Ultraviolet detector; Linearity; Quality control; Simultaneous measurement; Fat; Tocopherols; Nutrient; Carotenoid; Ultraviolet visible spectrometry
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-010-3655-2

Our aim was to assess the suitability of ultra-high performance liquid chromatography (UHPLC) for the simultaneous determination of biomarkers of vitamins A (retinol, retinyl esters), E (α- and γ-tocopherol), D (25-OH-vitamin D), and the major carotenoids in human serum to be used in clinical practice. UHPLC analysis was performed on HSS T3 column (2.1 × 100 mm; 1.8 μm) using gradient elution and UV-VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, α- and γ-tocopherol, lutein plus zeaxanthin, α-carotene, β-carotene, α- and β-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice.