Sox100B Regulates Progenitor-Specific Gene Expression and Cell Differentiation in the Adult Drosophila Intestine

• Published in: Stem cell reports Vol. 14; no. 2; pp. 226 - 240
• Main Authors: Meng, Fanju W., Rojas Villa, Sebastian E., Biteau, Benoît
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 11.02.2020; Elsevier
• Subjects: Aging - genetics; Animals; Base Sequence; Cell Differentiation; Cell Proliferation; differentiation; Drosophila; Drosophila melanogaster - cytology; Drosophila melanogaster - genetics; Drosophila Proteins - metabolism; Embryoid Bodies - cytology; Enhancer Elements, Genetic - genetics; Gene Expression Regulation, Developmental; Genes, Reporter; intestinal stem cells; Intestines - cytology; Introns - genetics; Sox100B; Sox21a; SOX9 Transcription Factor - metabolism; SOXB2 Transcription Factors - metabolism; Stem Cells - cytology; Stem Cells - metabolism; Sox21a; differentiation; Sox100B; Drosophila; intestinal stem cells
• ISSN: 2213-6711; 2213-6711
• DOI: 10.1016/j.stemcr.2020.01.003

Robust production of terminally differentiated cells from self-renewing resident stem cells is essential to maintain proper tissue architecture and physiological functions, especially in high-turnover tissues. However, the transcriptional networks that precisely regulate cell transition and differentiation are poorly understood in most tissues. Here, we identified Sox100B, a Drosophila Sox E family transcription factor, as a critical regulator of adult intestinal stem cell differentiation. Sox100B is expressed in stem and progenitor cells and required for differentiation of enteroblast progenitors into absorptive enterocytes. Mechanistically, Sox100B regulates the expression of another critical stem cell differentiation factor, Sox21a. Supporting a direct control of Sox21a by Sox100B, we identified a Sox21a intronic enhancer that is active in all intestinal progenitors and directly regulated by Sox100B. Taken together, our results demonstrate that the activity and regulation of two Sox transcription factors are essential to coordinate stem cell differentiation and proliferation and maintain intestinal tissue homeostasis. [Display omitted] •Sox100B is expressed in progenitor cells in the adult intestine•Sox100B is required for stem cell differentiation•Sox100B is required for Sox21a expression•Sox100B directly controls the activity of a Sox21a intronic enhancer In this article, Biteau and colleagues show that the transcription factor Sox100B is specifically expressed in stem cells and progenitors in the adult Drosophila intestinal epithelium. They demonstrate that it is essential for cell differentiation in the absorptive lineage and directly regulates the expression of the differentiation factor Sox21a.