A census of the lung: CellCards from LungMAP
The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to...
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Published in | Developmental cell Vol. 57; no. 1; pp. 112 - 145.e2 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
10.01.2022
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Subjects | |
Online Access | Get full text |
ISSN | 1534-5807 1878-1551 1878-1551 |
DOI | 10.1016/j.devcel.2021.11.007 |
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Abstract | The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health.
•CellCards provides a functional annotation of cell types within the lung•Combines functional and single cell data for a rigorous catalogue of cell lineages•CellCards will evolve to integrate and adapt to new data and emerging technologies
In Sun et al., the CellCards initiative integrates functional and emerging single cell genomic datasets for an accurate and evolvable annotation of important cell lineages within the respiratory system. Integration of functional characteristics is critical to properly delineate cell lineages at homeostasis and provide a baseline dataset for comparison to disease states. These CellCards will incorporate new and emerging datasets and technologies as they arise for a better understanding of the impact of acute and chronic disease on lung cell biology. |
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AbstractList | The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health.The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health. The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health. The human lung plays vital roles in respiration, host defense and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated from members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://lungmap.net/cell-cards-browser/ . We hope that this Lung CellCards will promote the community-wide effort to establish, maintain and restore respiratory health. Sun et al. takes a cellular census of the normal human lung by accounting for not only the recent explosion of single cell datasets, but also by annotating function and developmental origin. It is intended as a blueprint practical guide to initiate harmonization of cell nomenclature across lung biology studies. The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health. •CellCards provides a functional annotation of cell types within the lung•Combines functional and single cell data for a rigorous catalogue of cell lineages•CellCards will evolve to integrate and adapt to new data and emerging technologies In Sun et al., the CellCards initiative integrates functional and emerging single cell genomic datasets for an accurate and evolvable annotation of important cell lineages within the respiratory system. Integration of functional characteristics is critical to properly delineate cell lineages at homeostasis and provide a baseline dataset for comparison to disease states. These CellCards will incorporate new and emerging datasets and technologies as they arise for a better understanding of the impact of acute and chronic disease on lung cell biology. |
Author | Kyle, Jennifer Du, Yina Bardes, Eric E. Adkins, Joshua Pryhuber, Gloria Bell, Sheila M. Kalin, Tanya V. Basil, Maria C. Whitsett, Jeff Xu, Yan Clair, Geremy Zepp, Jarod A. Lin, Sara Guo, Minzhe Morley, Michael P. Aronow, Bruce J. Jin, Kang Bandyopadhyay, Gautam Zacharias, William J. Li, Rongbo Salomonis, Nathan Perl, Anne-Karina Wikenheiser-Brokamp, Kathryn A. Crotty Alexander, Laura E. Sajti, Eniko Ansong, Charles Deutsch, Gail Neithamer, Terren Kalinichenko, Vladimir V. Yu, Haoze V. Masso-Silva, Jorge A. Young, Randee Valdez, Michael J. Kitzmiller, Joseph A. Sun, Xin Misra, Ravi S. Verheyden, Jamie Deshmukh, Hitesh Morrisey, Edward E. |
AuthorAffiliation | 12 Department of Laboratories, Seattle Children’s Hospital, OC.8.720, 4800 Sand Point Way Northeast, Seattle, WA 98105 18 National Heart, Lung and Blood Institute, Bethesda, Maryland 9 Division of Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 15 Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 4 Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267 20 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104 1 Department of Pediatrics 13 Penn-CHOP Lung Biology Institute 7 Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington 16 Department of Pediatrics, Children’s Hospital of Philadelphia, Philadelphia, PA 19104 6 Department of Pediatrics Division of Neonatology, The University of Rochester Medical Cen |
AuthorAffiliation_xml | – name: 1 Department of Pediatrics – name: 2 Department of Biological Sciences, University of California at San Diego, 9500 Gilman Dr., La Jolla, CA 92093 – name: 18 National Heart, Lung and Blood Institute, Bethesda, Maryland – name: 17 Department of Internal Medicine, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267 – name: 15 Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH – name: 3 Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 – name: 20 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104 – name: 16 Department of Pediatrics, Children’s Hospital of Philadelphia, Philadelphia, PA 19104 – name: 5 Center for Lung Regenerative Medicine, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 – name: 4 Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267 – name: 13 Penn-CHOP Lung Biology Institute – name: 6 Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642 – name: 9 Division of Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 – name: 8 Deparment of Medicine, Division of Pulmonary, Critical Care, and Sleep Medicine, University of California San Diego, La Jolla, CA 92093 – name: 11 Department of Pathology, University of Washington School of Medicine, Seattle, WA – name: 14 Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104 – name: 19 Division of Biomedical Informatics, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio – name: 7 Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington – name: 10 Department of Pathology & Laboratory Medicine, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267 – name: 12 Department of Laboratories, Seattle Children’s Hospital, OC.8.720, 4800 Sand Point Way Northeast, Seattle, WA 98105 |
Author_xml | – sequence: 1 givenname: Xin surname: Sun fullname: Sun, Xin email: xinsun@health.ucsd.edu organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA – sequence: 2 givenname: Anne-Karina surname: Perl fullname: Perl, Anne-Karina organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 3 givenname: Rongbo surname: Li fullname: Li, Rongbo organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA – sequence: 4 givenname: Sheila M. surname: Bell fullname: Bell, Sheila M. organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 5 givenname: Eniko surname: Sajti fullname: Sajti, Eniko organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA – sequence: 6 givenname: Vladimir V. surname: Kalinichenko fullname: Kalinichenko, Vladimir V. organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 7 givenname: Tanya V. surname: Kalin fullname: Kalin, Tanya V. organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 8 givenname: Ravi S. surname: Misra fullname: Misra, Ravi S. organization: Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642, USA – sequence: 9 givenname: Hitesh surname: Deshmukh fullname: Deshmukh, Hitesh organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 10 givenname: Geremy surname: Clair fullname: Clair, Geremy organization: Biological Science Division, Pacific Northwest National Laboratory, Richland, WA, USA – sequence: 11 givenname: Jennifer surname: Kyle fullname: Kyle, Jennifer organization: Biological Science Division, Pacific Northwest National Laboratory, Richland, WA, USA – sequence: 12 givenname: Laura E. surname: Crotty Alexander fullname: Crotty Alexander, Laura E. organization: Deparment of Medicine, Division of Pulmonary, Critical Care, and Sleep Medicine, University of California, San Diego, La Jolla, CA 92093, USA – sequence: 13 givenname: Jorge A. surname: Masso-Silva fullname: Masso-Silva, Jorge A. organization: Deparment of Medicine, Division of Pulmonary, Critical Care, and Sleep Medicine, University of California, San Diego, La Jolla, CA 92093, USA – sequence: 14 givenname: Joseph A. surname: Kitzmiller fullname: Kitzmiller, Joseph A. organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 15 givenname: Kathryn A. surname: Wikenheiser-Brokamp fullname: Wikenheiser-Brokamp, Kathryn A. organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 16 givenname: Gail surname: Deutsch fullname: Deutsch, Gail organization: Department of Pathology, University of Washington School of Medicine, Seattle, WA, USA – sequence: 17 givenname: Minzhe surname: Guo fullname: Guo, Minzhe organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 18 givenname: Yina surname: Du fullname: Du, Yina organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 19 givenname: Michael P. surname: Morley fullname: Morley, Michael P. organization: Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA – sequence: 20 givenname: Michael J. surname: Valdez fullname: Valdez, Michael J. organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA – sequence: 21 givenname: Haoze V. surname: Yu fullname: Yu, Haoze V. organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA – sequence: 22 givenname: Kang surname: Jin fullname: Jin, Kang organization: Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA – sequence: 23 givenname: Eric E. surname: Bardes fullname: Bardes, Eric E. organization: Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA – sequence: 24 givenname: Jarod A. surname: Zepp fullname: Zepp, Jarod A. organization: Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA – sequence: 25 givenname: Terren surname: Neithamer fullname: Neithamer, Terren organization: Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA – sequence: 26 givenname: Maria C. surname: Basil fullname: Basil, Maria C. organization: Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA – sequence: 27 givenname: William J. surname: Zacharias fullname: Zacharias, William J. organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 28 givenname: Jamie surname: Verheyden fullname: Verheyden, Jamie organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA – sequence: 29 givenname: Randee surname: Young fullname: Young, Randee organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA – sequence: 30 givenname: Gautam surname: Bandyopadhyay fullname: Bandyopadhyay, Gautam organization: Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642, USA – sequence: 31 givenname: Sara surname: Lin fullname: Lin, Sara organization: National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA – sequence: 32 givenname: Charles surname: Ansong fullname: Ansong, Charles organization: Biological Science Division, Pacific Northwest National Laboratory, Richland, WA, USA – sequence: 33 givenname: Joshua surname: Adkins fullname: Adkins, Joshua organization: Biological Science Division, Pacific Northwest National Laboratory, Richland, WA, USA – sequence: 34 givenname: Nathan surname: Salomonis fullname: Salomonis, Nathan organization: Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267, USA – sequence: 35 givenname: Bruce J. surname: Aronow fullname: Aronow, Bruce J. organization: Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA – sequence: 36 givenname: Yan surname: Xu fullname: Xu, Yan organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 37 givenname: Gloria surname: Pryhuber fullname: Pryhuber, Gloria organization: Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642, USA – sequence: 38 givenname: Jeff surname: Whitsett fullname: Whitsett, Jeff organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA – sequence: 39 givenname: Edward E. surname: Morrisey fullname: Morrisey, Edward E. email: emorrise@pennmedicine.upenn.edu organization: Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34936882$$D View this record in MEDLINE/PubMed |
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Copyright | 2022 The Authors Copyright © 2021 Elsevier Inc. All rights reserved. |
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Keywords | lung COVID repair single cell disease LungMAP resource regeneration cell cards CellCards |
Language | English |
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Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 Conceptualization: X.S., E.E.M, J.W., Y,X., G.P., J.A., G.C., C.A., B.A.,N.S. Writing: X.S., A.K.P., R.L., S.M.B., E.S., V.V.K., T.V.K., R.S.M. H.D., G.C, L.E.C.A., J.M.S., J.A.Z., T.N., Y.X., E.E.M. Resources: G.P., R.S.M. Project administration: S.L. Funding acquisition: X.S., E.E.M, J.W., Y,X., G.P., J.A., G.C., C.A., B.A., N.S. Visualization: S.M.B., E.S., V.V.K, T.V.K., R.S.M., J.A..K., K.A.W.B., G.D., K.J. AUTHOR CONTRIBUTIONS Investigation: R.L., J.K., J.M.S., J.A.K., M.J.V., H.V.Y., K.J., J.A.Z., T.N., M.C.B., W.J.Z., J.V., R.Y. Data Curation: M.G., Y.D., M.P.M., M.J.V., H.V.Y., K.J., E.E.B., G.B. |
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PublicationTitle | Developmental cell |
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Snippet | The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and... The human lung plays vital roles in respiration, host defense and basic physiology. Recent technological advancements such as single-cell RNA sequencing and... |
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SubjectTerms | cell cards Cell Differentiation - genetics CellCards COVID Databases as Topic disease Humans lung Lung - cytology Lung - metabolism Lung - physiology LungMAP regeneration Regeneration - genetics repair resource single cell Single-Cell Analysis - methods |
Title | A census of the lung: CellCards from LungMAP |
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