A census of the lung: CellCards from LungMAP

The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to...

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Published inDevelopmental cell Vol. 57; no. 1; pp. 112 - 145.e2
Main Authors Sun, Xin, Perl, Anne-Karina, Li, Rongbo, Bell, Sheila M., Sajti, Eniko, Kalinichenko, Vladimir V., Kalin, Tanya V., Misra, Ravi S., Deshmukh, Hitesh, Clair, Geremy, Kyle, Jennifer, Crotty Alexander, Laura E., Masso-Silva, Jorge A., Kitzmiller, Joseph A., Wikenheiser-Brokamp, Kathryn A., Deutsch, Gail, Guo, Minzhe, Du, Yina, Morley, Michael P., Valdez, Michael J., Yu, Haoze V., Jin, Kang, Bardes, Eric E., Zepp, Jarod A., Neithamer, Terren, Basil, Maria C., Zacharias, William J., Verheyden, Jamie, Young, Randee, Bandyopadhyay, Gautam, Lin, Sara, Ansong, Charles, Adkins, Joshua, Salomonis, Nathan, Aronow, Bruce J., Xu, Yan, Pryhuber, Gloria, Whitsett, Jeff, Morrisey, Edward E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 10.01.2022
Subjects
Online AccessGet full text
ISSN1534-5807
1878-1551
1878-1551
DOI10.1016/j.devcel.2021.11.007

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Abstract The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health. •CellCards provides a functional annotation of cell types within the lung•Combines functional and single cell data for a rigorous catalogue of cell lineages•CellCards will evolve to integrate and adapt to new data and emerging technologies In Sun et al., the CellCards initiative integrates functional and emerging single cell genomic datasets for an accurate and evolvable annotation of important cell lineages within the respiratory system. Integration of functional characteristics is critical to properly delineate cell lineages at homeostasis and provide a baseline dataset for comparison to disease states. These CellCards will incorporate new and emerging datasets and technologies as they arise for a better understanding of the impact of acute and chronic disease on lung cell biology.
AbstractList The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health.The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health.
The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health.
The human lung plays vital roles in respiration, host defense and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated from members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://lungmap.net/cell-cards-browser/ . We hope that this Lung CellCards will promote the community-wide effort to establish, maintain and restore respiratory health. Sun et al. takes a cellular census of the normal human lung by accounting for not only the recent explosion of single cell datasets, but also by annotating function and developmental origin. It is intended as a blueprint practical guide to initiate harmonization of cell nomenclature across lung biology studies.
The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health. •CellCards provides a functional annotation of cell types within the lung•Combines functional and single cell data for a rigorous catalogue of cell lineages•CellCards will evolve to integrate and adapt to new data and emerging technologies In Sun et al., the CellCards initiative integrates functional and emerging single cell genomic datasets for an accurate and evolvable annotation of important cell lineages within the respiratory system. Integration of functional characteristics is critical to properly delineate cell lineages at homeostasis and provide a baseline dataset for comparison to disease states. These CellCards will incorporate new and emerging datasets and technologies as they arise for a better understanding of the impact of acute and chronic disease on lung cell biology.
Author Kyle, Jennifer
Du, Yina
Bardes, Eric E.
Adkins, Joshua
Pryhuber, Gloria
Bell, Sheila M.
Kalin, Tanya V.
Basil, Maria C.
Whitsett, Jeff
Xu, Yan
Clair, Geremy
Zepp, Jarod A.
Lin, Sara
Guo, Minzhe
Morley, Michael P.
Aronow, Bruce J.
Jin, Kang
Bandyopadhyay, Gautam
Zacharias, William J.
Li, Rongbo
Salomonis, Nathan
Perl, Anne-Karina
Wikenheiser-Brokamp, Kathryn A.
Crotty Alexander, Laura E.
Sajti, Eniko
Ansong, Charles
Deutsch, Gail
Neithamer, Terren
Kalinichenko, Vladimir V.
Yu, Haoze V.
Masso-Silva, Jorge A.
Young, Randee
Valdez, Michael J.
Kitzmiller, Joseph A.
Sun, Xin
Misra, Ravi S.
Verheyden, Jamie
Deshmukh, Hitesh
Morrisey, Edward E.
AuthorAffiliation 12 Department of Laboratories, Seattle Children’s Hospital, OC.8.720, 4800 Sand Point Way Northeast, Seattle, WA 98105
18 National Heart, Lung and Blood Institute, Bethesda, Maryland
9 Division of Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229
15 Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH
4 Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267
20 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104
1 Department of Pediatrics
13 Penn-CHOP Lung Biology Institute
7 Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington
16 Department of Pediatrics, Children’s Hospital of Philadelphia, Philadelphia, PA 19104
6 Department of Pediatrics Division of Neonatology, The University of Rochester Medical Cen
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– name: 18 National Heart, Lung and Blood Institute, Bethesda, Maryland
– name: 17 Department of Internal Medicine, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267
– name: 15 Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH
– name: 3 Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229
– name: 20 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104
– name: 16 Department of Pediatrics, Children’s Hospital of Philadelphia, Philadelphia, PA 19104
– name: 5 Center for Lung Regenerative Medicine, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229
– name: 4 Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267
– name: 13 Penn-CHOP Lung Biology Institute
– name: 6 Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642
– name: 9 Division of Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229
– name: 8 Deparment of Medicine, Division of Pulmonary, Critical Care, and Sleep Medicine, University of California San Diego, La Jolla, CA 92093
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– name: 14 Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104
– name: 19 Division of Biomedical Informatics, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio
– name: 7 Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington
– name: 10 Department of Pathology & Laboratory Medicine, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267
– name: 12 Department of Laboratories, Seattle Children’s Hospital, OC.8.720, 4800 Sand Point Way Northeast, Seattle, WA 98105
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  organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
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  givenname: Sheila M.
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  givenname: Tanya V.
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  givenname: Ravi S.
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– sequence: 12
  givenname: Laura E.
  surname: Crotty Alexander
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– sequence: 14
  givenname: Joseph A.
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  givenname: Gail
  surname: Deutsch
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  givenname: Yina
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  organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
– sequence: 19
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  givenname: Michael J.
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  givenname: Maria C.
  surname: Basil
  fullname: Basil, Maria C.
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– sequence: 27
  givenname: William J.
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– sequence: 29
  givenname: Randee
  surname: Young
  fullname: Young, Randee
  organization: Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
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  givenname: Gautam
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  organization: Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642, USA
– sequence: 31
  givenname: Sara
  surname: Lin
  fullname: Lin, Sara
  organization: National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
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– sequence: 33
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  organization: Biological Science Division, Pacific Northwest National Laboratory, Richland, WA, USA
– sequence: 34
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  surname: Salomonis
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  givenname: Bruce J.
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  organization: Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642, USA
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  givenname: Jeff
  surname: Whitsett
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  organization: Division of Neonatology and Pulmonary Biology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
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  givenname: Edward E.
  surname: Morrisey
  fullname: Morrisey, Edward E.
  email: emorrise@pennmedicine.upenn.edu
  organization: Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/34936882$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords lung
COVID
repair
single cell
disease
LungMAP
resource
regeneration
cell cards
CellCards
Language English
License This is an open access article under the CC BY license.
Copyright © 2021 Elsevier Inc. All rights reserved.
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content type line 23
Conceptualization: X.S., E.E.M, J.W., Y,X., G.P., J.A., G.C., C.A., B.A.,N.S.
Writing: X.S., A.K.P., R.L., S.M.B., E.S., V.V.K., T.V.K., R.S.M. H.D., G.C, L.E.C.A., J.M.S., J.A.Z., T.N., Y.X., E.E.M.
Resources: G.P., R.S.M.
Project administration: S.L.
Funding acquisition: X.S., E.E.M, J.W., Y,X., G.P., J.A., G.C., C.A., B.A., N.S.
Visualization: S.M.B., E.S., V.V.K, T.V.K., R.S.M., J.A..K., K.A.W.B., G.D., K.J.
AUTHOR CONTRIBUTIONS
Investigation: R.L., J.K., J.M.S., J.A.K., M.J.V., H.V.Y., K.J., J.A.Z., T.N., M.C.B., W.J.Z., J.V., R.Y.
Data Curation: M.G., Y.D., M.P.M., M.J.V., H.V.Y., K.J., E.E.B., G.B.
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PublicationTitle Developmental cell
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Snippet The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and...
The human lung plays vital roles in respiration, host defense and basic physiology. Recent technological advancements such as single-cell RNA sequencing and...
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SubjectTerms cell cards
Cell Differentiation - genetics
CellCards
COVID
Databases as Topic
disease
Humans
lung
Lung - cytology
Lung - metabolism
Lung - physiology
LungMAP
regeneration
Regeneration - genetics
repair
resource
single cell
Single-Cell Analysis - methods
Title A census of the lung: CellCards from LungMAP
URI https://dx.doi.org/10.1016/j.devcel.2021.11.007
https://www.ncbi.nlm.nih.gov/pubmed/34936882
https://www.proquest.com/docview/2613295524
https://pubmed.ncbi.nlm.nih.gov/PMC9202574
Volume 57
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