Pharmacological safety of Plinia cauliflora (Mart.) Kausel in rabbits

• Published in: Toxicology reports Vol. 6; pp. 616 - 624
• Main Authors: Palozi, Rhanany Alan Calloi, Guarnier, Lucas Pires, Romão, Paulo Vitor Moreira, Nocchi, Samara Requena, dos Santos, Carlos Calixto, Lourenço, Emerson Luiz Botelho, Silva, Denise Brentan, Gasparotto, Francielly Mourão, Gasparotto Junior, Arquimedes
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.01.2019; Elsevier
• Subjects: Cardiovascular; Irwin test; Myrtaceae; Respiratory; Toxicology; BB; LL; BE; Cardiovascular; ABG; H; Cl; CNS; ctCO2 (P); Na₂CO; K; tHb; PO2; Toxicology; ASE; DBP; PCO2; ANOVA; LC-DAD-MS; SO2; pH; Myrtaceae; ctO2; SBP; HHb; BEecf; ECG; GAE; P50; Respiratory; Ca+; Irwin test; S.E.M; RA; UFLC; EEPC; LA; cHCO3; Hct; Na+; O2Hb; RL; ctCO2 (B); MAP; BB, Buffer Base; Na₂CO₃, Sodium carbonate; P50, Half of the maximum hemoglobin saturation; H+, Hydrogen ion dissociated; ASE, Accelerated solvent extraction; O2Hb, Oxyhemoglobin; SO2, Level of hemoglobin-saturation by oxygen; tHb, Hemoglobin; Cl, Chloride; ECG, Electrocardiography; HHb, Deoxyhemoglobin; Hct, Hematocrit; BE, Base Excess; ctO2, Concentration of total oxygen; pH, Potential of hydrogen; LC-DAD-MS, Liquid chromatography coupled to a diode array detector and mass spectrometer; RA, Right arm; S.E.M, Standard error of the mean; SBP, Systolic blood pressure; CNS, Central nervous system; DBP, Diastolic blood pressure; PCO2, Partial pressure of carbon dioxide; MAP, Mean arterial pressure; ABG, Arterial blood gas; ANOVA, One-way analysis of variance; cHCO3, Bicarbonate concentration; ctCO2 (P), Concentration of total carbon dioxide in plasma; LA, Left arm; GAE, Gallic acid equivalent; Na+, Sodium; ctCO2 (B), Concentration of total carbon dioxide of whole blood; EEPC, Ethanol extract of Plinia cauliflora; PO2, Partial pressure of oxygen; Ca++, Calcium; LL, Left leg; BEecf, Base excess in the extracellular fluid compartment; RL, Right leg; UFLC, Ultra fast liquid chromatograph; K+, Potassium
• ISSN: 2214-7500; 2214-7500
• DOI: 10.1016/j.toxrep.2019.06.017

[Display omitted] •Fruit peels of Plinia cauliflora are widely used in Brazilian traditional medicine.•No studies have proved the safety of its pharmacological effects.•We assessed the safety pharmacology of P. cauliflora extract (EEPC) in rabbits.•EEPC did not cause any significant changes in several physiological systems.•These data provide important safety data for its clinical use. Fruit peels of Plinia cauliflora (Mart.) Kausel are widely used in Brazilian traditional medicine, but no studies have proved the safety of its pharmacological effects on the respiratory, cardiovascular, and central nervous systems. The present study assessed the safety pharmacology of P. cauliflora in New Zealand rabbits. First, an ethanol extract (EEPC) was selected for the pharmacological experiments and chemical characterization. Then, different groups of rabbits were orally treated with EEPC (200 and 2000 mg/kg) or vehicle. Acute behavioral and physiological alterations in the modified Irwin test, respiratory rate, arterial blood gas, and various cardiovascular parameters (i.e., heart rate, blood pressure, and electrocardiography) were evaluated. The main secondary metabolites that were identified in EEPC were ellagic acid, gallic acid, O-deoxyhexosyl quercetin, and the anthocyanin O-hexosyl cyanidin. No significant behavioral or physiological changes were observed in any of the groups. None of the doses of EEPC affected respiratory rate or arterial blood gas, with no changes on blood pressure or electrocardiographic parameters. The present study showed that EEPC did not cause any significant changes in respiratory, cardiovascular, or central nervous system function. These data provide scientific evidence of the effects of this species and important safety data for its clinical use.