Unstable simple volatiles and gas chromatography-tandem mass spectrometry analysis of essential oil from the roots bark of Oplopanax horridus extracted by supercritical fluid extraction

• Published in: Molecules (Basel, Switzerland) Vol. 19; no. 12; pp. 19708 - 19717
• Main Authors: Shao, Li, Bao, Mei-Hua, Ouyang, Dong-Sheng, Wang, Chong-Zhi, Yuan, Chun-Su, Zhou, Hong-Hao, Huang, Wei-Hua
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.11.2014; MDPI
• Subjects: (S,E)-nerolidol; Chromatography; Chromatography, Supercritical Fluid - methods; Efficiency; Gas Chromatography-Mass Spectrometry - methods; GC/MS; Mass spectrometry; Oils & fats; Oils, Volatile - chemistry; Oils, Volatile - isolation & purification; Oplopanax - chemistry; Oplopanax horridus; Plant Bark - chemistry; Plant Roots - chemistry; Pressure; Reproducibility of Results; S-falcarinol; Scientific imaging; SFE; Tandem Mass Spectrometry - methods; Temperature; Time Factors; volatile oil; North America; China
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules191219708

Volatile oil from the root bark of Oplopanax horridus is regarded to be responsible for the clinical uses of the title plant as a respiratory stimulant and expectorant. Therefore, a supercritical fluid extraction method was first employed to extract the volatile oil from the roots bark of O. horridus, which was subsequently analyzed by GC/MS. Forty-eight volatile compounds were identified by GC/MS analysis, including (S,E)-nerolidol (52.5%), τ-cadinol (21.6%) and S-falcarinol (3.6%). Accordingly, the volatile oil (100 g) was subjected to chromatographic separation and purification. As a result, the three compounds, (E)-nerolidol (2 g), τ-cadinol (62 mg) and S-falcarinol (21 mg), were isolated and purified from the volatile oil, the structures of which were unambiguously elucidated by detailed spectroscopic analysis including 1D- and 2D-NMR techniques.