Multiplexed detection of protein cancer markers on Au/Ag-barcoded nanorods using fluorescent-conjugated polymers

• Published in: Analytical and bioanalytical chemistry Vol. 397; no. 6; pp. 2261 - 2270
• Main Authors: Zheng, Weiming, He, Lin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.07.2010; Springer-Verlag; Springer
• Subjects: Analytical Chemistry; Animals; Automatic Data Processing; Bar codes; Biochemistry; Biomarkers, Tumor - analysis; Cancer; Cancer marker detection; Cattle; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Diagnosis; DNA; Encoded nanoparticle; Exact sciences and technology; Fluorescence; Fluorescent Dyes; Fluorescent-conjugated polymer; Food Science; Gold; Humans; Immunoassay - methods; Laboratory Medicine; Limit of Detection; Markers; Mathematical analysis; Miscellaneous; Monitoring/Environmental Analysis; Multiplexing; Nanorods; Nanotubes - chemistry; Neoplasm Proteins - analysis; Paper in Forefront; Polymers; Protein binding; Protein sensing; Proteins; Sensitivity and Specificity; Silver; Solution array; Spectrometric and optical methods; Multiplexing; Fluorescent-conjugated polymer; Protein sensing; Cancer marker detection; Encoded nanoparticle; Solution array; Nanoparticle; Fluorescence; Probe; Fluorescence spectrometry; Immunological method; Molecules; Target; Double stranded DNA; Specificity; Serum; Ungulata; Bovine; Antibody; Sample; Use; Polymer; Marker; Protein; Antigen; Array; Vertebrata; Mammalia; Simultaneous measurement; Artiodactyla; Detection
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-010-3834-1

Integration of fluorescent-conjugated polymers as detection moiety with metallic striped nanorods for multiplexed detection of clinically important cancer marker proteins in an immunoassay format was demonstrated in this report. Specifically, cationic conjugated polymers were introduced to protein complexes through electrostatic binding to negatively charged double-stranded DNA, which was tagged on detection antibodies prior to antigen recognition. The intense fluorescence emission of conjugated polymers resulted in highly sensitive detection of cancer marker proteins wherein an undiluted bovine serum sample as low as ∼25 target molecules captured on each particle was detectable. Meanwhile, the use of polymer molecules as the detection probe did not obscure the optical pattern of underlying nanorods, i.e., the encoding capability of barcoded nanorods was preserved, which allowed simultaneous detection of three cancer marker proteins with good specificity.