Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy

• Published in: Cell reports (Cambridge) Vol. 5; no. 1; pp. 151 - 165
• Main Authors: Shcherbakova, Inna, Hoskins, Aaron A., Friedman, Larry J., Serebrov, Victor, Corrêa, Ivan R., Xu, Ming-Qun, Gelles, Jeff, Moore, Melissa J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 17.10.2013; Elsevier
• Subjects: Binding Sites; Humans; Introns; Microscopy, Fluorescence - methods; RNA Precursors - genetics; RNA Precursors - metabolism; RNA Splicing; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - analysis; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Spliceosomes - chemistry; Spliceosomes - genetics; Spliceosomes - metabolism
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2013.08.026

Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable recruitment of U1 small nuclear ribonucleoprotein particle (snRNP) to the 5′ splice site necessarily precedes recruitment of U2 snRNP to the branch site to form the “prespliceosome.” Here, using colocalization single-molecule spectroscopy to follow initial spliceosome assembly on eight different S. cerevisiae pre-mRNAs, we demonstrate that active yeast spliceosomes can form by both U1-first and U2-first pathways. Both assembly pathways yield prespliceosomes functionally equivalent for subsequent U5⋅U4/U6 tri-snRNP recruitment and for intron excision. Although fractional flux through the two pathways varies on different introns, both are operational on all introns studied. Thus, multiple pathways exist for assembling functional spliceosomes. These observations provide insight into the mechanisms of cross-intron coordination of initial spliceosome assembly. [Display omitted] •Functional spliceosomes can assemble via both U1- and U2-first pathways•The first U1 binding event does not commit a pre-mRNA to splicing•Splicing rates and efficiencies are not limited by subcomplex association rates•An updated spliceosome cycle is proposed Intron excision by the spliceosome is an essential process in eukaryotic gene expression. Gelles, Moore, and colleagues use single-molecule colocalization to monitor early spliceosome assembly events in yeast whole-cell extract. They demonstrate that pre-mRNAs can initiate the formation of functional spliceosomes by first binding either U1 or U2 snRNP. This branched pathway has important implications for mechanisms of cross-intron coordination during early spliceosome assembly.